Anticorps Polyclonal de lapin anti-Paxillin

Paxillin Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

15

Avis (2)

N° de cat : 10029-1-Ig

Synonymes

paxillin, PXN

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Galerie de données de validation

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  WB analysis of mouse brain using 10029-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10029-1-Ig (PXN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 10029-1-Ig

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 10029-1-Ig (Paxillin Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 10029-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10029-1-Ig (PXN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10029-1-Ig

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10029-1-Ig (PXN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 10029-1-Ig

  IP Result of anti-PXN (IP:10029-1-Ig, 3ug; Detection:10029-1-Ig 1:1000) with mouse brain tissue lysate 7500ug.

• 

  IHC staining of human liver cancer using 10029-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 10029-1-Ig(PXN antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 10029-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 10029-1-Ig(PXN antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 10029-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10029-1-Ig (Paxillin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HUVEC using 10029-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using Paxillin antibody (10029-1-Ig) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). DAPI (blue).

• 

  IF Staining of HepG2 using 10029-1-Ig

  Immunofluorescent analysis of -20℃ Ethanol fixed HepG2 cells using 10029-1-Ig (Paxillin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). The cytoskeleton was labelled in red with 66031-1-Ig (alpha tubulin). DAPI (blue).

• 

  IF Staining of HepG2 using 10029-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 10029-1-Ig (Paxillin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of NIH/3T3 using 10029-1-Ig

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed NIH/3T3 cells using 10029-1-Ig(Paxillin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules COLO 320, cellules Jurkat, cellules MCF-7
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer du foie humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HUVEC, cellules HepG2, cellules NIH/3T3

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 9 publications below
• IHC: See 1 publications below
• IF: See 7 publications below

Informations sur le produit

10029-1-Ig cible Paxillin dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: paxillin
• Masse moléculaire calculée: 68 kDa
• Poids moléculaire observé: 68 kDa
• Numéro d’acquisition GenBank: NM_002859
• Symbole du gène: PXN
• Identification du gène (NCBI): 5829
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PXN (paxillin) is a 68 kDa scaffold protein that interacts with multiple structural and signaling proteins and regulates cell adhesion, migration, proliferation, and apoptosis. PXN is thought to play an important role in tumor migration, invasion, and metastasis (21045234). PXN has been identified as a direct substrate of protein tyrosine phosphatase receptor-type T (PTPRT), a potent tumor suppressor gene. Increased phospho-PXN at tyrosine residue 88 (Y88) has been found as a common feature of human colon cancers (20133777).

Publications

Species	Application	Title
human	WB	Mol Cell; Direct epitranscriptomic regulation of mammalian translation initiation through N4-acetylcytidine.; Authors - Daniel Arango
human	IF	Bone Res; Impairment of rigidity sensing caused by mutant TP53 gain of function in osteosarcoma; Authors - Ming Luo
human	IF	Cell Rep; Mechanotransduction in response to ECM stiffening impairs cGAS immune signaling in tumor cells; Authors - Yingqi Liu
human	WB	Cell Death Dis; ZC3H15 promotes glioblastoma progression through regulating EGFR stability.; Authors - Jianbing Hou
human	IF	Acta Pharmacol Sin; Reduced intracellular chloride concentration impairs angiogenesis by inhibiting oxidative stress-mediated VEGFR2 activation.; Authors - Kai Li
human	IF	Sci Signal; Mitotic cell responses to substrate topological cues are independent of the molecular nature of adhesion.; Authors - Ouranio Anastasiou

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Thomas (Verified Customer) (09-22-2022)

Works in WB (pulldown via PAK1) and IF (costained with F-Actin in cyan)

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:100 (IF), 1:500 (WB)
• Cell Tissue Type: Human Brain Microvascular endothelial cell line

Paxillin Antibody Western Blot,Immunofluorescence validation (1:100 (IF), 1:500 (WB) dilution) in Human Brain Microvascular endothelial cell line (Cat no:10029-1-Ig)

FH

Boyan (Verified Customer) (03-11-2019)

For WB, besides the expected band, it also recognised some other non-specific bands; for IF, it could label the cell cortex localisation, partially co-localised with Actin.

• Applications: Western Blot, Immunofluorescence,
• Cell Tissue Type: MEF

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