Anticorps Monoclonal anti-Pan Acetylation

Pan Acetylation Monoclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

chemical compound et plus (5)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA, Acetylation assay

Conjugaison

Non conjugué

Publications(52)

CloneNo.

1D5F2

Avis (1)

N° de cat : 66289-1-Ig

Synonymes

Pan Acetyl, Pan-Acetyl, Acetylation, Acetylation Lysine, Acetylated Lysine

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Galerie de données de validation

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  WB analysis of HeLa using 66289-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66289-1-Ig (Pan Acetylation Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 66289-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 66289-1-Ig (Pan Acetylation antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of MDCK using 66289-1-Ig

  Immunofluorescent analysis of (10% Formaldehyde) fixed MDCK cells using 66289-1-Ig (Pan Acetylation antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa
• Résultats positifs en IF/ICC: cellules HeLa, cellules MDCK

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 39 publications below
• IHC: See 2 publications below
• IF: See 4 publications below
• IP: See 3 publications below
• CoIP: See 5 publications below

Informations sur le produit

66289-1-Ig cible Pan Acetylation dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA, Acetylation assay et montre une réactivité avec des échantillons chemical compound

• Réactivité: chemical compound
• Réactivité citée: rat, Drosophile, Humain, riz, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: Pan Acetylation
• Poids moléculaire observé: 17 kDa
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Acetylation and De-acetylation are the important reaction in epigenetic. Many proteins, especially Histone, are with Acetylated of Lysine. This antibody is used to detect the Acetylation of Lysine. The 17 kDa in our detection is Acetylated Histone.

Publications

Species	Application	Title
mouse	WB,IF	Mol Cell; Serine synthesis sustains macrophage IL-1β production via NAD+-dependent protein acetylation; Authors - Chuanlong Wang
mouse	WB,IF	Neuron; Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory.; Authors - Xiaohui Li
		Sci Adv; Asparagine transporter supports macrophage inflammation via histone phosphorylation; Authors - Chuanlong Wang
Drosophila	WB	Dev Cell; HDAC6 Suppresses Age-Dependent Ectopic Fat Accumulation by Maintaining the Proteostasis of PLIN2 in Drosophila.; Authors - Yan Yan
human	WB	Cell Death Dis; RSL1D1 promotes the progression of colorectal cancer through RAN-mediated autophagy suppression.; Authors - Xunhua Liu
rice	Acetylation assay	Plant Biotechnol J; The rice RNase P protein subunit Rpp30 confers broad-spectrum resistance to fungal and bacterial pathogens.; Authors - Wei Li

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Xiaoyu (Verified Customer) (07-18-2024)

Gives a nice, strong and specific signal in Western Blot.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: 293T

Pan Acetylation Antibody Western Blot validation (1:1000 dilution) in 293T (Cat no:66289-1-Ig)

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