Anticorps Polyclonal de lapin anti-Phospho-CREB1 (Ser133)

Phospho-CREB1 (Ser133) Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(44)

N° de cat : 28792-1-AP

Synonymes

CREB1 (Ser133), p CREB1, p CREB1 (Ser133), p CREB1 Ser133, p-CREB1

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Galerie de données de validation

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  WB analysis using 28792-1-AP

  Non-treated and Forskolin treated HSC-T6 cells were subjected to SDS PAGE followed by western blot with 28792-1-AP (Phospho-CREB1 (Ser133) antibody) at dilution of 1:4000 incubated at room temperature for 1 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

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  WB analysis using 28792-1-AP

  Non-treated and Anisomycin treated HeLa cells were subjected to SDS PAGE followed by western blot with 28792-1-AP (Phospho-CREB1 (Ser133) antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  WB analysis using 28792-1-AP

  Non-treated and Forskolin treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 28792-1-AP (Phospho-CREB1 (Ser133) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 28792-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 28792-1-AP (Phospho-CREB1 (Ser133) antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HSC-T6 traitées à la forskoline, cellules HeLa, cellules NIH/3T3
• Résultats positifs en IHC: tissu hépatique humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 42 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

28792-1-AP cible Phospho-CREB1 (Ser133) dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: cAMP responsive element binding protein 1
• Masse moléculaire calculée: 341 aa, 35 kDa
• Poids moléculaire observé: 35-46 kDa
• Numéro d’acquisition GenBank: BC010636
• Symbole du gène: CREB1
• Identification du gène (NCBI): 1385
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The cAMP-response element binding protein (CREB) is localized in the nucleus and acts as a transcription factor, which binds to the cAMP response element (CRE) of the promoters of its target genes, upon phosphorylation at Ser133 by different receptor-activated protein kinases, such as protein kinase A (PKA), calmodulin-dependent protein kinase (CaMK), mitogen-activated protein kinases (MAPK), and other kinases. The activity of CREB1 in neurons has been correlated with various intracellular processes, including proliferation, differentiation, survival, long-term synaptic potentiation, neurogenesis, and neuronal plasticity. CREB1 belongs to the CREB/activating transcription factor (ATF) family of transcription factors. (PMID: 30214393, PMID: 33917483, PMID: 32605164) The antibody also detects the phosphorylated form of the CREB-related protein ATF1.

Publications

Species	Application	Title
mouse	WB	Free Radic Biol Med; Reprimo (RPRM) mediates neuronal ferroptosis via CREB-Nrf2/SCD1 pathways in radiation-induced brain injury; Authors - Wenyu Shi
human	WB	FASEB J; Ketone body 3-hydroxybutyrate mitigates apoptosis and enhances osteogenesis in bone organoid construction via the cAMP/PKA/CREB signaling pathway; Authors - Songlun Jiang
human	WB	J Ethnopharmacol; Yangjing Zhongyu decoction facilitates mitochondrial activity, estrogenesis, and energy metabolism in H2O2-induced human granulosa cell line KGN.; Authors - Jia Liu
mouse	WB	Brain Res Bull; Metabotropic glutamate receptor 5 upregulation of γ-aminobutyric acid transporter 3 expression ameliorates cognitive impairment after traumatic brain injury in mice.; Authors - Peng Xu
mouse	WB	Int J Biol Macromol; Lactoferrin ameliorates cognitive impairment in D-galactose-induced aging mice by regulating the PI3K/Akt/mTOR signaling pathway and the microbiome-gut-brain axis; Authors - Mengqi Wang
rat	WB	Phytomedicine; Peiyuan Ningshen-ointment alleviates learning and memory impairment induced by sleep deprivation through activating the cAMP/PKA signaling pathway; Authors - Xiao-Yu Wei

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