Anticorps Polyclonal de lapin anti-Phospho-Chk1 (Ser345)

Phospho-Chk1 (Ser345) Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(5)

N° de cat : 28803-1-AP

Synonymes

CHEK1, CHK1, CHK1 checkpoint homolog, Chk 1, CHEK 1

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Galerie de données de validation

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  WB analysis using 28803-1-AP

  Non-treated and Calyculin A treated cells were subjected to SDS PAGE followed by western blot with 28803-1-AP (Phospho-Chk1 (Ser345) antibody) at dilution of 1:1000 incubated at room temperature for 1 hours.

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  IF Staining of HeLa using 28803-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Calyculin A treated HeLa cells using Phospho-Chk1 (Ser345) antibody (28803-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules NIH/3T3 traitées à la calyculine A, cellules HeLa traitées à la calyculine A
• Résultats positifs en IF/ICC: cellules HeLa traitées à la calyculine A,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below

Informations sur le produit

28803-1-AP cible Phospho-Chk1 (Ser345) dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: CHK1 checkpoint homolog (S. pombe)
• Masse moléculaire calculée: 54 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC004202
• Symbole du gène: Chk1
• Identification du gène (NCBI): 1111
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The checkpoint kinase 1 (Chk1) is a conserved kinase that imposes cell cycle delays in response to impediments to DNA replication. Chk1 kinase is downstream of the ATR kinase. ATR phosphorylates Chk1 kinase and other proteins to ensure replication is being blocked as to avoid replication fork collapse and DNA damage. Activation of Chk1 involves phosphorylation at Ser317 and Ser345 by ATM/ATR, followed by autophosphorylation of Ser296. In vitro Chk1 binds to and phosphorylate the dual-specificity protein phosphatases Cdc25A, Cdc25B, and Cdc25C, which control cell cycle transitions by dephosphorylating cyclin-dependent kinases. (PMID:22941630, PMID: 32571801, PMID:19276361)

Publications

Species	Application	Title
human	WB	Transl Oncol; The ATR inhibitor VE-821 increases the sensitivity of gastric cancer cells to cisplatin; Authors - Haochen Su
human	WB	Phytomedicine; Epiberberine induced p53/p21-dependent G2/M cell cycle arrest and cell apoptosis in gastric cancer cells by activating γ-aminobutyric acid receptor- β3; Authors - Mengmeng Li
human	WB	J Exp Clin Cancer Res; HIF-1α-HPRT1 axis promotes tumorigenesis and gefitinib resistance by enhancing purine metabolism in EGFR-mutant lung adenocarcinoma; Authors - Pengyu Geng
human	WB	Cancer Sci; P2RY6 deletion promotes UVB-induced skin carcinogenesis by activating the PI3K/AKT signal pathway; Authors - Peng Xu
human	WB	Bioorg Chem; Structurally diverse tetrahydroxanthone analogues from Paraconiothyrium sp. AC31 with pyroptosis induction through targeted inhibition of PARP1 in hepatocellular carcinoma cells; Authors - Meng-Ke Zhang

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