Anticorps Polyclonal de lapin anti-Phospho-GYS (Ser641)

Phospho-GYS (Ser641) Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(1)

N° de cat : 28855-1-AP

Synonymes

GYS2, p GYS1 , p GYS1 (Ser641), p GYS1 Ser641, p-GYS1

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Galerie de données de validation

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  WB analysis using 28855-1-AP

  Non-treated PC-3 cells, phosphatase inhibitor treated and λ phosphatase PC-3 cells were subjected to SDS PAGE followed by western blot with 28855-1-AP (Phospho-GYS1 (Ser641) antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

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  IF Staining of PC-3 using 28855-1-AP

  Immunofluorescent analysis of (4% PFA) fixed λ phosphatase treated PC-3 cells using Phospho-GYS1 (Ser641) antibody (28855-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: λ phosphatase treated PC-3 cells,
• Résultats positifs en IF/ICC: λ phosphatase treated PC-3 cells,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

28855-1-AP cible Phospho-GYS (Ser641) dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: glycogen synthase 1 (muscle)
• Masse moléculaire calculée: 84 kDa
• Poids moléculaire observé: 84 kDa
• Numéro d’acquisition GenBank: BC007688
• Symbole du gène: GYS1
• Identification du gène (NCBI): 2997
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Glycogen synthase 1 (GYS1, GS) catalyzes the key step of glycogen synthesis and plays an important role in glycogen metabolism in the liver and muscle. In kidney tissues, glycogen synthase 1 (GYS1) is the most important rate-limiting enzyme functioning in the last step of glycogen synthesis. Pathologically, its deficiency has been shown to cause muscle glycogen storage disease type 0 and death. Studies of tumors showed that GYS1 was rapidly induced under hypoxic conditions and positively correlated with glycogen accumulation in glioblastoma, breast, and colon cancer cell lines. GYS1 is phosphorylated at nine sites, and insulin stimulates the dephosphorylation of glycogen synthase. Insulin stimulates dephosphorylation of glycogen synthase via PKB-mediated phosphorylation of GSK3. Phosphorylation of GSK3 decreases kinase activity, which will decrease phosphorylation of GS and increase glycogen synthase fractional activity. (PMID: 32802186, PMID: 30443599, PMID:22232606)

Publications

Species	Application	Title
mouse	WB	J Ethnopharmacol; Si-Ni-San promotes liver regeneration by maintaining hepatic oxidative equilibrium and glucose/lipid metabolism homeostasis; Authors - Xu Yang

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