Anticorps Polyclonal de lapin anti-Phospho-MEK2 (Thr394)

Phospho-MEK2 (Thr394) Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications(5)

N° de cat : 28955-1-AP

Synonymes

ERK activator kinase 2, FLJ26075, MAP kinase kinase 2, MAP2K2, MAPK/ERK kinase 2, MAPKK 2, MAPKK2, MEK 2, MEK2, MKK2, Phospho-MAP2K2 (Thr394), PRKMK2

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Galerie de données de validation

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  WB analysis using 28955-1-AP

  Non-treated NIH/3T3, phosphatase inhibitor treated and λ phosphatase treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 28955-1-AP (Phospho-MEK2 (Thr394) antibody) at dilution of 1:1000 incubated at 4℃ overnight.

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Applications testées

• Résultats positifs en WB: cellules NIH/3T3, cellules NIH/3T3 traitées à la λ phosphatase

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IF: See 1 publications below

Informations sur le produit

28955-1-AP cible Phospho-MEK2 (Thr394) dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: mitogen-activated protein kinase kinase 2
• Masse moléculaire calculée: 44 kDa
• Poids moléculaire observé: 44 kDa
• Numéro d’acquisition GenBank: BC018645
• Symbole du gène: MEK2
• Identification du gène (NCBI): 5605
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MEK2(MAPK/ERK kinase 2) is also named as MAP2K2, MKK2, PRKMK2 and belongs to the MAP kinase kinase subfamily. MAPKK is itself dependent on Ser/Thr phosphorylation for activity catalyzed by MAP kinase kinase kinases (RAF or MEKK1). MEK1 and MEK2 are closely related, dual-specificity tyrosine/threonine protein kinases found in the Ras/Raf/MEK/ERK mitogen-activated protein kinase (MAPK) signaling pathway.

Publications

Species	Application	Title
mouse	WB,IF	Nat Immunol; Defining blood-induced microglia functions in neurodegeneration through multiomic profiling; Authors - Andrew S Mendiola
rat	WB	Pharm Biol; Myrtenol improves brain damage and promotes angiogenesis in rats with cerebral infarction by activating the ERK1/2 signalling pathway.; Authors - Shengming Huang
human	WB	Int J Biol Sci; Dual role of CD73 as a signaling molecule and adenosine-generating enzyme in colorectal cancer progression and immune evasion; Authors - Weidong Lian
human	WB	Cell Mol Biol Lett; A specific super-enhancer actuated by berberine regulates EGFR-mediated RAS-RAF1-MEK1/2-ERK1/2 pathway to induce nasopharyngeal carcinoma autophagy; Authors - Yao Wu
human	WB	Mol Med Rep; Downregulation of microRNA‑221‑3p promotes angiogenesis of lipoprotein(a)‑injured endothelial progenitor cells by targeting silent information regulator 1 to activate the RAF/MEK/ERK signaling pathway; Authors - Xiaolei Zhang

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