Anticorps Polyclonal de lapin anti-Phospho-PPP1CA (Thr320)

Phospho-PPP1CA (Thr320) Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications(1)

N° de cat : 29874-1-AP

Synonymes

Phospho-PPP1CA (Thr320), PP 1A, PPP1A, PPP1CA

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Galerie de données de validation

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  WB analysis using 29874-1-AP

  Non-treated and Calyculin A treated various cells were subjected to SDS PAGE followed by western blot with 29874-1-AP (Phospho-PPP1CA (Thr320) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as the loading control.

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Applications testées

• Résultats positifs en WB: cellules HeLa traitées à la calyculine A, cellules Jurkat traitées à la calyculine A

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

29874-1-AP cible Phospho-PPP1CA (Thr320) dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: protein phosphatase 1, catalytic subunit, alpha isoform
• Masse moléculaire calculée: 330 aa, 38 kDa
• Poids moléculaire observé: 37 kDa
• Numéro d’acquisition GenBank: BC001888
• Symbole du gène: PPP1CA
• Identification du gène (NCBI): 5499
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Protein phosphatase associates with over 200 regulatory proteins to form highly specific holoenzymes which dephosphorylate hundreds of biological targets. Type 1 protein phosphatase (PP1), a serine/threonine phosphatase, is essential for cell division and participates in the regulation of glycogen metabolism, muscle contractility, and protein synthesis. Four isoforms of PP1 have been characterized: PP1α, PP1δ, PP1γ1 and PP1γ2 (PMID: 9835651). It has been illustrated that PP1 dephosphorylates Rb and cdc25 during mitosis (PMID: 8384581, PMID: 1392080). A cell cycle-dependent phosphorylation at Thr320 of PP1α by cdc2 kinase inhibits PP1α activity (PMID: 9122166).

Publications

Species	Application	Title
mouse	WB	Nat Commun; Inhibited peroxidase activity of peroxiredoxin 1 by palmitic acid exacerbates nonalcoholic steatohepatitis in male mice; Authors - Wen Yin

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