Anticorps Polyclonal de lapin anti-Phospho-RB1 (Ser807/811)

Phospho-RB1 (Ser807/811) Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications(8)

N° de cat : 30376-1-AP

Synonymes

OSRC, p105 Rb, Phospho-RB1 (Ser807/811), pp110, pRb, RB, RB1, retinoblastoma 1

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Galerie de données de validation

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  WB analysis using 30376-1-AP

  Non-treated and Calyculin A treated Jurkat cells were subjected to SDS PAGE followed by western blot with 30376-1-AP (Phospho-RB1 (Ser807/811) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with RB1 antibody (10048-2-Ig) subsequently.

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Applications testées

• Résultats positifs en WB: cellules Jurkat traitées à la calyculine A, cellules Jurkat

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 7 publications below

Informations sur le produit

30376-1-AP cible Phospho-RB1 (Ser807/811) dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: retinoblastoma 1
• Masse moléculaire calculée: 928 aa, 106 kDa
• Poids moléculaire observé: 110 kDa
• Numéro d’acquisition GenBank: BC039060
• Symbole du gène: RB1
• Identification du gène (NCBI): 5925
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RB1, also named as pp110, pRb and p105 Rb, belongs to the retinoblastoma protein (RB) family. Phosphorylation and dephosphorylation of RB1 is well known to be a key regulator in cell cycle progression in cancer cells. Phosphorylation of RB1 at multiple S/T residues, first by cyclin D-dependent kinases and subsequently by cyclin E-CDK2 in later G1 phase, completely inactivates RB1, enabling the release of E2F from RB1 complexes and activation of E2F-mediated transcription. Monomethylation at Lys810 by SMYD2 enhances phosphorylation at Ser807 and Ser811, and promotes cell cycle progression. (PMID: 22787429, PMID: 21115810, PMID: 32344731)

Publications

Species	Application	Title
human		Cell Death Discov; Mitochondrial priming and response to BH3 mimetics in "one-two punch" senogenic-senolytic strategies; Authors - Júlia López
mouse	WB	Int J Mol Sci; Arthrocolin B Impairs Adipogenesis via Delaying Cell Cycle Progression During the Mitotic Clonal Expansion Period; Authors - Guang Cao
	WB	EMBO Mol Med; Exploiting metabolic vulnerability in glioblastoma using a brain-penetrant drug with a safe profile; Authors - Audrey Burban
human	WB	Phytother Res; Inhibition of Hepatoblastoma by Ganoderma Lucidum Polysaccharide via the Induction of HDAC4-p16-RB Axis-Mediated Cellular Senescence; Authors - Ting Ye
human	WB	Biomedicines; Mitophagy-Mediated Tumor Dormancy Protects Cancer Cells from Chemotherapy; Authors - Yunqing Sun
human	WB	Front Pharmacol; Network pharmacology and molecular docking reveal the mechanisms of curcumin activity against esophageal squamous cell carcinoma; Authors - Jian Wang

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