Anticorps Polyclonal de lapin anti-Phospho-S6 Ribosomal protein (Ser235/236)

Phospho-S6 Ribosomal protein (Ser235/236) Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications(12)

Avis (2)

N° de cat : 29223-1-AP

Synonymes

p S6Ribosomal protein, p S6Ribosomal protein (Ser235/236), p S6Ribosomal protein Ser235/236, Phospho RPS6, Phospho RPS6 (Ser235/236)

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Galerie de données de validation

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  WB analysis using 29223-1-AP

  Non-treated NIH/3T3, phosphatase inhibitor treated and λ phosphatase treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 29223-1-AP (Phospho-S6 Ribosomal protein (Ser235/236) antibody) at dilution of 1:1000 incubated at room temperature for 1 hours.

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  WB analysis using 29223-1-AP

  Non-treated NIH/3T3 and PDGF (HZ-1215) treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 29223-1-AP (Phospho-S6 Ribosomal protein (Ser235/236) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules NIH/3T3 traitées au PDGF, cellules NIH/3T3 traitées à la λ phosphatase

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 11 publications below
• IF: See 1 publications below

Informations sur le produit

29223-1-AP cible Phospho-S6 Ribosomal protein (Ser235/236) dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: ribosomal protein S6
• Masse moléculaire calculée: 29 kDa
• Poids moléculaire observé: 32 kDa
• Numéro d’acquisition GenBank: BC000524
• Symbole du gène: RPS6
• Identification du gène (NCBI): 6194
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Ribosomal protein S6 (RPS6),Phosphoprotein NP33.It may play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest. Phosphorylated at Ser-235 and Ser-236 by RPS6KA1 and RPS6KA3; phosphorylation at these sites facilitates the assembly of the preinitiation complex.

Publications

Species	Application	Title
Human	WB	Sci Rep; STK11 genetic alterations in metastatic EGFR mutant lung cancer; Authors - Dandan Yin
mouse	WB	ACS Omega; ROS-responsive MSC-derived Exosome Mimetics Carrying MHY1485 Alleviate Renal Ischemia Reperfusion Injury through Multiple Mechanisms; Authors - Zhiyu Qian
human	WB	Int J Med Sci; PRMT4 promotes hepatocellular carcinoma progression by activating AKT/mTOR signaling and indicates poor prognosis.; Authors - Peng Du
	WB	Metabolites; mTORC1 Mediates the Processes of Lysine Regulating Satellite Cells Proliferation, Apoptosis, and Autophagy; Authors - Mengqi Liu
human	WB	Aging (Albany NY); RIOK3 promotes mTORC1 activation by facilitating SLC7A2-mediated arginine uptake in pancreatic ductal adenocarcinoma; Authors - Henan Qin
mouse	IF	Exp Neurol; Synergistic effect of chemogenetic activation of corticospinal motoneurons and physical exercise in promoting functional recovery after spinal cord injury; Authors - Xueling Lin

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Morgane (Verified Customer) (09-15-2025)

Very good anti-phospho-antibody, very specific to RPS6

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HCT116

Phospho-S6 Ribosomal protein (Ser235/236) Antibody Western Blot validation (1/1000 dilution) in HCT116 (Cat no:29223-1-AP)

FH

Reyes (Verified Customer) (02-14-2025)

pS6 worked perfectly (in green) showing the mTOR activation in my neurons (in red NeuN) and Balloon Cells on human brain FFPE tissue.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Human Brain Cortex

Phospho-S6 Ribosomal protein (Ser235/236) Antibody Immunofluorescence validation (1:100 dilution) in Human Brain Cortex (Cat no:29223-1-AP)

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