Anticorps Recombinant de lapin anti-Phospho-SMAD1 (Ser214)

Phospho-SMAD1 (Ser214) Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

3K8

N° de cat : 81433-1-RR

Synonymes

BSP 1, BSP1, hSMAD1, JV4 1, JV41, MAD homolog 1, Mad related protein 1, MADH1, MADR1, Mothers against DPP homolog 1, Phospho-SMAD1 (Ser214), SMAD 1, SMAD family member 1, SMAD1

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Galerie de données de validation

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  WB analysis of A431 using 81433-1-RR

  Non-treated and UV treated A431 cells were subjected to SDS PAGE followed by western blot with 81433-1-RR (Phospho-SMAD1 (Ser214) antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

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  WB analysis of HepG2 using 81433-1-RR

  Non-treated and Calyculin A treated HepG2 cells were subjected to SDS PAGE followed by western blot with 81433-1-RR (Phospho-SMAD1 (Ser214) antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

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  WB analysis of NIH/3T3 using 81433-1-RR

  Non-treated and UV treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 81433-1-RR (Phospho-SMAD1 (Ser214) antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

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Applications testées

• Résultats positifs en WB: cellules A431 traitées aux UV, cellules HepG2 traitées à la calyculine A, cellules NIH/3T3 traitées au UV

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

81433-1-RR cible Phospho-SMAD1 (Ser214) dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: SMAD family member 1
• Masse moléculaire calculée: 52 kDa
• Poids moléculaire observé: 52 kDa
• Numéro d’acquisition GenBank: BC001878
• Symbole du gène: SMAD1
• Identification du gène (NCBI): 4086
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SMAD family member 1 (Smad1) have been involved in metastatic progression of many cancer types. Smad1 can be induced by many tumor-stimulating cytokines such as the bone morphogenetic protein 2 (BMP2) and TNFα and plays important roles in cell invasion and metastasis. BMP2 signalling is initiated by binding to its specific receptors, which leads to in the phosphorylation and nuclear translocation of Smad1. Translocated Smad1 then modulates the expression of downstream osteogenic genes. (PMID: 32954678, PMID: 30008908)