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Anticorps Polyclonal de lapin anti-Phospho-SPHK1 (Ser225)

Phospho-SPHK1 (Ser225) Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(16)

N° de cat : 19561-1-AP

Synonymes

Phospho-SPHK1 (Ser225), SK 1, sphingosine kinase 1, SPHK, SPHK1, SPHK1 phopho, SPHK1-Phospho-Ser225, SPK, SPK 1



Applications testées

Résultats positifs en WBcellules L02, tissu testiculaire humain

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

19561-1-AP cible Phospho-SPHK1 (Ser225) dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

Réactivité Humain
Réactivité citéerat, Humain, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène Peptide
Nom complet sphingosine kinase 1
Masse moléculaire calculée 51 kDa
Poids moléculaire observé 45-47 kDa
Numéro d’acquisition GenBankNM_182965
Symbole du gène SPHK1
Identification du gène (NCBI) 8877
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS with 0.02% sodium azide and 50% glycerol
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SPHK1, also named as SPHK and SPK, catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. SPHK1 acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D, L-threo-dihydrosphingosine, N, N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol. The antibody is specific to Ser(311)-phospho of SPHK1(isoform2) or Ser(225)-phospho of SPHK1(isoform1).

Protocole

Product Specific Protocols
WB protocol for Phospho-SPHK1 (Ser225) antibody 19561-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Cell Death Differ

Cell fate determined by the activation balance between PKR and SPHK1.

Authors - Han Qiao
humanWB

Ecotoxicol Environ Saf

Combined effect of co-exposure to di (2-ethylhexyl) phthalates and 50-Hz magnetic-fields on promoting human amniotic cells proliferation.

Authors - Liangjing Chen
humanWB

Ecotoxicol Environ Saf

A 50-Hz magnetic-field exposure promotes human amniotic cells proliferation via SphK-S1P-S1PR cascade mediated ERK signaling pathway.

Authors - Liangjing Chen
mouseWB

Biochim Biophys Acta Mol Basis Dis

Involvement of released sphingosine 1-phosphate/sphingosine 1-phosphate receptor axis in skeletal muscle atrophy.

Authors - Federica Pierucci
human,ratWB,IHC

Phytomedicine

Geniposide alleviates VEGF-induced angiogenesis by inhibiting VEGFR2/PKC/ERK1/2-mediated SphK1 translocation.

Authors - Yan Wang
ratWB

Phytother Res

Geniposide downregulates the VEGF/SphK1/S1P pathway and alleviates angiogenesis in rheumatoid arthritis in vivo and in vitro.

Authors - Yan Wang
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