Anticorps Polyclonal de lapin anti-Phospho-SPHK1 (Ser225)

• Phare
• Validé par KD/KO

Phospho-SPHK1 (Ser225) Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

13

N° de cat : 19561-1-AP

Synonymes

Phospho-SPHK1 (Ser225), SK 1, sphingosine kinase 1, SPHK, SPHK1, SPHK1 phopho, SPHK1-Phospho-Ser225, SPK, SPK 1

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Galerie de données de validation

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  WB analysis of L02 using 19561-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 19561-1-AP (SPHK1-Phospho-Ser225 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 19561-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 19561-1-AP (SPHK1-Phospho-Ser225 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 19561-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 19561-1-AP (SPHK1-Phospho-Ser225 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules L02, tissu testiculaire humain

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 11 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

19561-1-AP cible Phospho-SPHK1 (Ser225) dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: sphingosine kinase 1
• Masse moléculaire calculée: 51 kDa
• Poids moléculaire observé: 45-47 kDa
• Numéro d’acquisition GenBank: NM_182965
• Symbole du gène: SPHK1
• Identification du gène (NCBI): 8877
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SPHK1, also named as SPHK and SPK, catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. SPHK1 acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D, L-threo-dihydrosphingosine, N, N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol. The antibody is specific to Ser(311)-phospho of SPHK1(isoform2) or Ser(225)-phospho of SPHK1(isoform1).

Publications

Species	Application	Title
human	IF	Cell Death Differ; Cell fate determined by the activation balance between PKR and SPHK1.; Authors - Han Qiao
human	WB	Ecotoxicol Environ Saf; Combined effect of co-exposure to di (2-ethylhexyl) phthalates and 50-Hz magnetic-fields on promoting human amniotic cells proliferation.; Authors - Liangjing Chen
human	WB	Ecotoxicol Environ Saf; A 50-Hz magnetic-field exposure promotes human amniotic cells proliferation via SphK-S1P-S1PR cascade mediated ERK signaling pathway.; Authors - Liangjing Chen
mouse	WB	Biochim Biophys Acta Mol Basis Dis; Involvement of released sphingosine 1-phosphate/sphingosine 1-phosphate receptor axis in skeletal muscle atrophy.; Authors - Federica Pierucci
human,rat	WB,IHC	Phytomedicine; Geniposide alleviates VEGF-induced angiogenesis by inhibiting VEGFR2/PKC/ERK1/2-mediated SphK1 translocation.; Authors - Yan Wang
rat	WB	Phytother Res; Geniposide downregulates the VEGF/SphK1/S1P pathway and alleviates angiogenesis in rheumatoid arthritis in vivo and in vitro.; Authors - Yan Wang