Anticorps Recombinant de lapin anti-Phospho-TAU (Ser404)

Phospho-TAU (Ser404) Recombinant Antibody for WB, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF-P, ELISA

Conjugaison

Non conjugué

Publications(5)

CloneNo.

5I16

Avis (1)

N° de cat : 81383-1-RR

Synonymes

p TAU, p TAU (Ser404), p TAU Ser404, Phospho TAU, Phospho TAU (Ser404)

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Galerie de données de validation

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  WB analysis of mouse brain using 81383-1-RR

  Phosphatase inhibitor treated and λ phosphatase treated mouse brain tissue were subjected to SDS PAGE followed by western blot with 81383-1-RR (Phospho-TAU (Ser404) antibody) at dilution of 1:16000 incubated at room temperature for 1 hours. The membrane was stripped and re-blotted with Beta Tubulin antibody as loading control.

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  IF Staining of mouse brain using 81383-1-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded λ phosphatase treated mouse brain tissue using Phospho-TAU (Ser404) antibody (81383-1-RR, Clone: 5I16 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de souris
• Résultats positifs en IF-P: tissu cérébral de souris

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IF: See 2 publications below

Informations sur le produit

81383-1-RR cible Phospho-TAU (Ser404) dans les applications de WB, IF-P, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: microtubule-associated protein tau
• Masse moléculaire calculée: 37-46, 79-81 kDa
• Poids moléculaire observé: 45-55 kDa
• Numéro d’acquisition GenBank: BC000558
• Symbole du gène: TAU
• Identification du gène (NCBI): 4137
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Tau (tubulin-associated unit) is a microtubule-associated protein (also known as MAPT), expressed mainly in neurons of the central nervous system. Its primary function is to modulate microtubule dynamics for maintaining axonal cytoskeleton. The Tau protein has six isoforms produced from a single gene through alternative RNA splicing. Isoforms differ in number of inserts at the N-terminal half and the number of repeats at the C-terminal half (3 repeat-3R; 4 repeat-4R). Tau is hyperphosphorylated during aging and in age-related neurodegenerative diseases such as Alzheimer's disease (AD) and fronto-temporal dementia. Hyperphosphorylation of Tau leads to the formation of neurofibrillary tangles (NFT) in the neurons and glia cells, which is one of the hallmarks of AD.

Publications

Species	Application	Title
mouse	WB	Neuromolecular Med; Lycium Barbarum Polysaccharides Improves Cognitive Functions in ICV-STZ-Induced Alzheimer's Disease Mice Model by Improving the Synaptic Structural Plasticity and Regulating IRS1/PI3K/AKT Signaling Pathway; Authors - Yingxi He
mouse	WB	Foods; Comparative Analysis of Egg Yolk Phospholipid Unsaturation and Its Impact on Neural Health in Alzheimer Disease Mice; Authors - Yuhang Sun
mouse	WB,IF	Redox Biol; Absence of astrocytic ceruloplasmin reverses the senescence process with aging of learning and memory abilities; Authors - Zhong-Da Li
mouse	WB,IF	Neurochem Int; Suppression of KDM5C mitigates the symptoms of Alzheimer's disease by up-regulating BDNF expression; Authors - Jingjing Han
mouse,human	WB	J Med Chem; Discovery of Effective Dual PROTAC Degraders for Neurodegenerative Disease-Associated Aggregates; Authors - Wentao Zhu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Marion (Verified Customer) (12-09-2024)

DAPI (blue) & pTau Ser404 (red) This antibody was tested to reveal Tau phosporylation in a murine AD model. The antibody works fine for IF on mouse tissue.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1/400
• Cell Tissue Type: Mouse Brain

Phospho-TAU (Ser404) Antibody Immunofluorescence validation (1/400 dilution) in Mouse Brain (Cat no:81383-1-RR)

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