Anticorps Monoclonal anti-Protein A

Protein A Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Staphylococcus aureus et plus (3)

Applications

WB, IP, ELISA

Conjugaison

Non conjugué

Publications(4)

CloneNo.

1G6A6

N° de cat : 66945-1-Ig

Synonymes

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Galerie de données de validation

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  WB analysis of Recombinant protein using 66945-1-Ig

  Commercial Protein A was deactivated and lysised followed by SDS-PAGE and then blot with 66945-1-Ig (Protein A antibody) at dilution of 1:10000 and isotype control antibody 66360-1-Ig at 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of ProteinA using 66945-1-Ig

  Commercial Protein A and negative samples (human plasma, HeLa cells, rat liver, mouse brain) were subjected to SDS-PAGE followed by western blot with 66945-1-Ig (Protein A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: Protéine recombinante, Protéine recombinante

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

66945-1-Ig cible Protein A dans les applications de WB, IP, ELISA et montre une réactivité avec des échantillons Staphylococcus aureus

• Réactivité: Staphylococcus aureus
• Réactivité citée: Humain, levure, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: Protein A
• Numéro d’acquisition GenBank: EU695225
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Protein A is a surface protein of S.aureus which binds IgG molecules by their Fc region (PMID: 4163007). Engineered Protein A has been widely used in antibody purification due to its Fc binding property. However, trace of protein A might be leaked from purification resin thus causes contamination of final products. Antibody against Protein A or ELISA kits for Protein A could be used to evaluate leaking level of Protein A from resin. Protein A should be deactivated (e.g. boiling in 5-10% tween-20) before testing.

Publications

Species	Application	Title
	IP	Virus Res; HA2 from the Helicoverpa armigera nucleopolyhedrovirus: a WASP-related protein that activates Arp2/3-induced actin filament formation.; Authors - Qian Wang
human		J Biochem; Title: Cloaking the ACE2 receptor with salivary cationic proteins inhibits SARS-CoV-2 entry.; Authors - Katsutoshi Yoshizato
mouse	WB	Hepatology; Concerted synergy between viral-specific IgG and CD8 + T cells is critical for clearance of an HCV-related rodent hepacivirus; Authors - John Gridley
yeast	WB	Proc Natl Acad Sci U S A; DNA polymerase delta governs parental histone transfer to DNA replication lagging strand; Authors - Congcong Tian

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