Anticorps Monoclonal anti-RAB11A

RAB11A Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications(3)

CloneNo.

4A4C9

Avis (1)

N° de cat : 67902-1-Ig

Synonymes

Rab 11, RAB11, RAB11A/B, 4A4C9, EC:3.6.5.2

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Galerie de données de validation

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  WB analysis using 67902-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67902-1-Ig (RAB11A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  WB analysis using 67902-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67902-1-Ig (RAB11A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  WB analysis of mouse brain using 67902-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 67902-1-Ig (RAB11A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67902-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67902-1-Ig (RAB11A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67902-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67902-1-Ig (RAB11A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat cerebellum using 67902-1-Ig

  rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 67902-1-Ig (RAB11A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 67902-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 67902-1-Ig (RAB11A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of A549 using 67902-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using RAB11A antibody (67902-1-Ig, Clone: 4A4C9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

• 

  FC experiment of HeLa using 67902-1-Ig

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug RAB11A Monoclonal antibody (67902-1-Ig, Clone:4A4C9) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules A431, cellules A549, cellules HeLa, cellules HepG2, cellules K-562, tissu cérébral de lapin, tissu cérébral de porc, tissu cérébral de rat, tissu de cervelet de rat
• Résultats positifs en IF/ICC: cellules A549,
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:20000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

67902-1-Ig cible RAB11A dans les applications de WB, IHC, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: RAB11A Protéine recombinante Ag8879
• Nom complet: RAB11A, member RAS oncogene family
• Masse moléculaire calculée: 216 aa, 24 kDa
• Poids moléculaire observé: 24 kDa
• Numéro d’acquisition GenBank: BC013348
• Symbole du gène: RAB11A
• Identification du gène (NCBI): 8766
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Rab11a, Rab11b and Rab25 comprise a subfamily of Rab proteins that belong to the Ras-related small GTPase protein superfamily (PMID: 8074219, PMID: 10708602). Rab11 proteins are involved in endocytosis and plasma membrane recycling in non-polarized and polarized cells ( PMID: 8638719, PMID: 8922376). Rab11a and Rab11b are ubiquitously expressed in most tissues, while Rab25 is only expressed in epithelial cells. Rab11a is known to regulate the recycling of internalized cell surface proteins and receptors from the early endosome through the trans-Golgi network (PMID: 11121436, PMID: 9600939).

Publications

Species	Application	Title
	IHC	Nat Metab; Autophagy regulator ATG5 preserves cerebellar function by safeguarding its glycolytic activity; Authors - Janine Tutas
human	IF	Cell Rep; A MYC-STAMBPL1-TOE1 positive feedback loop mediates EGFR stability in hepatocellular carcinoma; Authors - Hongli Zhang
	WB,IF	Cell Commun Signal; Atg5-deficient mesenchymal stem cells protect against non-alcoholic fatty liver by accelerating hepatocyte growth factor secretion; Authors - Caifeng Zhang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

X (Verified Customer) (10-17-2024)

A very good antibody to detect endo Rab11A from several sources.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: mouse brain lysate

RAB11A Antibody Western Blot validation (1:2000 dilution) in mouse brain lysate (Cat no:67902-1-Ig)

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