Anticorps Polyclonal de lapin anti-RAB11B-Specific

RAB11B-Specific Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 19742-1-AP

Synonymes

GTP binding protein YPT3, H YPT3, RAB11B, RAB11B-Specific, Ras related protein Rab 11B, YPT3

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Galerie de données de validation

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  WB analysis of human brain using 19742-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 19742-1-AP (RAB11B-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 19742-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19742-1-AP (RAB11B-Specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 19742-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 19742-1-AP (RAB11B-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 19742-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 19742-1-AP (RAB11B-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-12 using 19742-1-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 19742-1-AP (RAB11B-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of C6 using 19742-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 19742-1-AP (RAB11B-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-12 using 19742-1-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 19742-1-AP (RAB11B-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 19742-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 19742-1-AP (RAB11B-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of fetal human brain using 19742-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 19742-1-AP (RAB11B-Specific Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 19742-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19742-1-AP (RAB11B-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 19742-1-AP

  IP result of anti-RAB11B-Specific (IP:19742-1-AP, 4ug; Detection:19742-1-AP 1:1000) with mouse brain tissue lysate 3200 ug.

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  IHC staining of human lung using 19742-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 19742-1-AP (RAB11B-Specific antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 19742-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 19742-1-AP (RAB11B-Specific antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human placenta using 19742-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 19742-1-AP (RAB11B-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 19742-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 19742-1-AP (RAB11B-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 19742-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 19742-1-AP (RAB11B-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 19742-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 19742-1-AP (RAB11B-Specific antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu cérébral humain, cellules C6, cellules HEK-293, cellules HeLa, cellules PC-12, tissu cérébral humain fœtal, tissu testiculaire de souris, tissu testiculaire humain
• Résultats positifs en IP: tissu cérébral de souris,
• Résultats positifs en IHC: tissu pulmonaire humain, tissu cérébral humain, tissu placentaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:300-1:600
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

19742-1-AP cible RAB11B-Specific dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, canin, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: RAB11B, member RAS oncogene family
• Masse moléculaire calculée: 24 kDa
• Poids moléculaire observé: 24 kDa
• Numéro d’acquisition GenBank: NM_004218
• Symbole du gène: RAB11B
• Identification du gène (NCBI): 9230
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RAB11B, also named as YPT3, belongs to the small GTPase superfamily and Rab family. RAB11B possesses GTPase activity. The antibody is specific to RAN11B. The antibody has no cross reaction to RAB11A.

Publications

Species	Application	Title
human	WB	Oncotarget; Upregulated long non-coding RNA AFAP1-AS1 expression is associated with progression and poor prognosis of nasopharyngeal carcinoma.; Authors - Hao Bo
human	WB	J Exp Clin Cancer Res; Long noncoding RNA AFAP1-AS1 acts as a competing endogenous RNA of miR-423-5p to facilitate nasopharyngeal carcinoma metastasis through regulating the Rho/Rac pathway.; Authors - Yu Lian
human	WB	Tumour Biol; AFAP1-AS1, a long noncoding RNA upregulated in lung cancer and promotes invasion and metastasis.; Authors - Zhaoyang Zeng
canine,rat	WB,IHC	J Histochem Cytochem; Canine Salivary Glands: Analysis of Rab and SNARE Protein Expression and SNARE Complex Formation With Diverse Tissue Properties.; Authors - Hiroshi Gomi
human	WB,IF	J Neurosci; The Late Endosomal Pathway Regulates the Ciliary Targeting of Tetraspanin Protein Peripherin 2.; Authors - Wataru Otsu