Anticorps Polyclonal de lapin anti-RAB11FIP1

RAB11FIP1 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, singe, souris

Applications

WB, IHC, IF, IP, RIP, ELISA

Conjugaison

Non conjugué

Publications(5)

N° de cat : 16778-1-AP

Synonymes

Rab-coupling protein, Rab11-FIP1, rab11 FIP1, Rab11 family-interacting protein 1, Rab coupling protein

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Galerie de données de validation

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  WB analysis of HepG2 using 16778-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16778-1-AP (RAB11FIP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COS-7 using 16778-1-AP

  COS-7 cells were subjected to SDS PAGE followed by western blot with 16778-1-AP (RAB11FIP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SKOV-3 using 16778-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 16778-1-AP (RAB11FIP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 16778-1-AP

  IP result of anti-RAB11FIP1 (IP:16778-1-AP, 4ug; Detection:16778-1-AP 1:600) with HepG2 cells lysate 3200ug.

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  IHC staining of human breast cancer using 16778-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16778-1-AP (RAB11FIP1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 16778-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16778-1-AP (RAB11FIP1 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 16778-1-AP

  Immunofluorescent analysis of HeLa cells using 16778-1-AP (RAB11FIP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules COS-7, cellules SKOV-3
• Résultats positifs en IP: cellules HepG2
• Résultats positifs en IHC: tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• RIP: See 2 publications below

Informations sur le produit

16778-1-AP cible RAB11FIP1 dans les applications de WB, IHC, IF, IP, RIP, ELISA et montre une réactivité avec des échantillons Humain, rat, singe, souris

• Réactivité: Humain, rat, singe, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RAB11FIP1 Protéine recombinante Ag10179
• Nom complet: RAB11 family interacting protein 1 (class I)
• Masse moléculaire calculée: 649aa,71 kDa; 1283aa,137 kDa
• Poids moléculaire observé: 71 kDa
• Numéro d’acquisition GenBank: BC077720
• Symbole du gène: RAB11FIP1
• Identification du gène (NCBI): 80223
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB,RIP	Mol Cancer; CircRTN4 promotes pancreatic cancer progression through a novel CircRNA-miRNA-lncRNA pathway and stabilizing epithelial-mesenchymal transition protein; Authors - Chi Hin Wong
human	WB	Brief Bioinform; mi-IsoNet: systems-scale microRNA landscape reveals rampant isoform-mediated gain of target interaction diversity and signaling specificity.; Authors - Li Guo
mouse	RIP	Cancer Res; CircFOXK2 Promotes Growth and Metastasis of Pancreatic Ductal Adenocarcinoma by Complexing with RNA Binding Proteins and Sponging MiR-942.; Authors - Chi Hin Wong
human	WB,IHC	Am J Cancer Res; In silico development and in vitro validation of a novel five-gene signature for prognostic prediction in colon cancer; Authors - Qiankun Zhu
rat	WB,IF	ACS Appl Mater Interfaces; Lithium Promotes Osteogenesis via Rab11a-Facilitated Exosomal Wnt10a Secretion and β-Catenin Signaling Activation; Authors - Changjun Chen

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