Anticorps Polyclonal de lapin anti-RAB11FIP2

• Phare
• Validé par KD/KO

RAB11FIP2 Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(4)

N° de cat : 18136-1-AP

Synonymes

NRip11, Rab11 family-interacting protein 2, Rab11 FIP2, Rab11-FIP2

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Galerie de données de validation

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  WB analysis using 18136-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18136-1-AP (RAB11FIP2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 18136-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 18136-1-AP (RAB11FIP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 18136-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 18136-1-AP (RAB11FIP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 18136-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RAB11FIP2 antibody (18136-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of HepG2 using 18136-1-AP

  Immunofluorescent analysis of HepG2 cells, using RAB11FIP2 antibody 18136-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).Blue pseudocolor = DAPI (fluorescent DNA dye).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules HEK-293, tissu cérébral de rat, tissu hépatique de souris
• Résultats positifs en IF/ICC: cellules HeLa, cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 2 publications below

Informations sur le produit

18136-1-AP cible RAB11FIP2 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RAB11FIP2 Protéine recombinante Ag12736
• Nom complet: RAB11 family interacting protein 2 (class I)
• Masse moléculaire calculée: 512 aa, 58 kDa
• Poids moléculaire observé: 58 kDa
• Numéro d’acquisition GenBank: BC075073
• Symbole du gène: RAB11FIP2
• Identification du gène (NCBI): 22841
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RAB11FIP2, or RAB11 family interacting protein 2, is a protein that plays a crucial role in intracellular trafficking, particularly in the recycling of endocytic vesicles. It is involved in several cellular processes, including TRAM-dependent toll-like receptor 4 signaling pathway, phagocytosis, and positive regulation of GTPase activity. RAB11FIP2 is localized in endosomes, nucleoplasm, and phagocytic cups, and it is expressed ubiquitously in various tissues, with higher expression in endometrium and brain. RAB11FIP2 interacts with the actin-based motor myo5b and the small GTPase Rab11, which are key components in the regulation of vesicle tethering and transport along actin filaments. This interaction is particularly important for processes such as cell polarization, motility, and lumen formation in multicellular structures. .

Publications

Species	Application	Title
human	WB,IF	PLoS Pathog; Acquisition of Rab11 and Rab11-Fip2-A novel strategy for Chlamydia pneumoniae early survival.; Authors - Katja Mölleken ; KD Validated
human	WB	Traffic; Rab11-FIP2 Interaction with MYO5B Regulates Movement of Rab11a-Containing Recycling Vesicles.; Authors - Jenny C Schafer
human	IF	Exp Cell Res; Rab11-FIP1A regulates early trafficking into the recycling endosomes.; Authors - Jenny C Schafer
human	WB	Cell Biosci; Peptides targeting RAB11A-FIP2 complex inhibit HPIV3, RSV, and IAV replication as broad-spectrum antivirals; Authors - Yanliang Jiang

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