Anticorps Polyclonal de lapin anti-RAB14

• Phare
• Validé par KD/KO

RAB14 Polyclonal Antibody for IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

12

N° de cat : 15662-1-AP

Synonymes

FBP, RAB 14, RAB14, Ras related protein Rab 14

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Galerie de données de validation

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  WB analysis of mouse brain using 15662-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15662-1-AP (RAB14 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 15662-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15662-1-AP (RAB14 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 15662-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15662-1-AP (RAB14 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 15662-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15662-1-AP (RAB14 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 15662-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 15662-1-AP (RAB14 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary tumor using 15662-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15662-1-AP (RAB14 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 15662-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15662-1-AP (RAB14 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules HepG2, cellules Raji, tissu cérébral humain, tissu rénal de souris
• Résultats positifs en IHC: tissu de tumeur ovarienne humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 10 publications below
• IHC: See 5 publications below
• IF: See 1 publications below

Informations sur le produit

15662-1-AP cible RAB14 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RAB14 Protéine recombinante Ag8092
• Nom complet: RAB14, member RAS oncogene family
• Masse moléculaire calculée: 215 aa, 24 kDa
• Poids moléculaire observé: 24 kDa
• Numéro d’acquisition GenBank: BC006081
• Symbole du gène: RAB14
• Identification du gène (NCBI): 51552
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB,IHC	Cell Physiol Biochem; Hypermethylation of miR-338-3p and Impact of its Suppression on Cell Metastasis Through N-Cadherin Accumulation at the Cell -Cell Junction and Degradation of MMP in Gastric Cancer.; Authors - Bo Guo
human	IHC,WB	Onco Targets Ther; Rab14 Overexpression Promotes Proliferation and Invasion Through YAP Signaling in Non-Small Cell Lung Cancers.; Authors - Jun Zhang
human	WB	PLoS One; Rab14 Act as Oncogene and Induce Proliferation of Gastric Cancer Cells via AKT Signaling Pathway.; Authors - Bo Guo
human	WB,IHC	Virchows Arch; PMID: 30267303; Authors - Jinnian Ge ; KD Validated
human	WB,IHC	Tumour Biol; Rab14 is overexpressed in ovarian cancers and promotes ovarian cancer proliferation through Wnt pathway.; Authors - Rui Hou ; KD Validated
human	WB	Eur Rev Med Pharmacol Sci; STAT3-induced upregulation of lncRNA DUXAP8 functions as ceRNA for miR-577 to promote the migration and invasion in colorectal cancer through the regulation of RAB14.; Authors - C Du