Anticorps Monoclonal anti-RAB1B

• Phare
• Validé par KD/KO

RAB1B Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

3C2G6

N° de cat : 67837-1-Ig

Synonymes

3C2G6, EC:3.6.5.2, Ras related protein Rab 1B, Ras-related protein Rab-1B

---

Galerie de données de validation

• 

  WB analysis using 67837-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67837-1-Ig (RAB1B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 67837-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67837-1-Ig (RAB1B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 67837-1-Ig

  WB result of RAB1B antibody (67837-1-Ig; 1:15000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAB1B transfected HeLa cells.

• 

  IHC staining of human lung cancer using 67837-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67837-1-Ig (RAB1B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 67837-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67837-1-Ig (RAB1B antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 67837-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67837-1-Ig (RAB1B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 67837-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67837-1-Ig (RAB1B antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat heart using 67837-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 67837-1-Ig (RAB1B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat heart using 67837-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 67837-1-Ig (RAB1B antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 67837-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67837-1-Ig (RAB1B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 67837-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67837-1-Ig (RAB1B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 67837-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67837-1-Ig (RAB1B antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 67837-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67837-1-Ig (RAB1B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 67837-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67837-1-Ig (RAB1B antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 67837-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67837-1-Ig (RAB1B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 67837-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using RAB1B antibody (67837-1-Ig, Clone: 3C2G6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of NIH/3T3 using 67837-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using RAB1B antibody (67837-1-Ig, Clone: 3C2G6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

---

Applications testées

• Résultats positifs en WB: cellules A549, cellules HEK-293, cellules HeLa, cellules HSC-T6, cellules Jurkat, cellules K-562, cellules LNCaP, cellules NIH/3T3, cellules U2OS
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu cardiaque de rat, tissu cérébral de rat, tissu de cancer de l'estomac humain, tissu de cancer du foie humain, tissu de cancer du pancréas humain, tissu de tumeur ovarienne humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules NIH/3T3, cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

67837-1-Ig cible RAB1B dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: RAB1B Protéine recombinante Ag12069
• Nom complet: RAB1B, member RAS oncogene family
• Masse moléculaire calculée: 201 aa, 22 kDa
• Poids moléculaire observé: 22 kDa
• Numéro d’acquisition GenBank: BC071169
• Symbole du gène: RAB1B
• Identification du gène (NCBI): 81876
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

{{ptg:RelatedPrimaryAntibodies}}