Anticorps Polyclonal de lapin anti-RAB22A

• Phare
• Validé par KD/KO

RAB22A Polyclonal Antibody for IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (3)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

22

N° de cat : 12125-1-AP

Synonymes

Rab 22, RAB22, RAB22A, Ras related protein Rab 22A

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Galerie de données de validation

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  WB analysis of COLO 320 using 12125-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 12125-1-AP (RAB22A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 12125-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12125-1-AP (RAB22A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of BxPC-3 using 12125-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 12125-1-AP (RAB22A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 12125-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 12125-1-AP (RAB22A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of COLO 320 using 12125-1-AP

  IP result of anti-RAB22A (IP:12125-1-AP, 4ug; Detection:12125-1-AP 1:300) with COLO 320 cells lysate 2560 ug.

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  IHC staining of human gliomas using 12125-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 12125-1-AP (RAB22A antibody) at dilution of 1:100 (under 10x lens).

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Applications testées

• Résultats positifs en WB: cellules COLO 320, cellules A375, cellules BxPC-3, cellules Jurkat
• Résultats positifs en IP: cellules COLO 320,
• Résultats positifs en IHC: tissu de gliome humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 15 publications below
• IHC: See 7 publications below
• IF: See 6 publications below

Informations sur le produit

12125-1-AP cible RAB22A dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: canin, Humain, souris, Hamster
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RAB22A Protéine recombinante Ag2769
• Nom complet: RAB22A, member RAS oncogene family
• Masse moléculaire calculée: 194 aa, 22 kDa
• Poids moléculaire observé: 22 kDa
• Numéro d’acquisition GenBank: BC015710
• Symbole du gène: RAB22A
• Identification du gène (NCBI): 57403
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RAB22A is a small GTPase that is expressed ubiquitously in mammalian tissues. It localizes in the MHCI-containing recycling endosomal tubules and functions in endocytic pathway. RAB22A may regulate the dynamic interactions of endosomal compartments and it may be involved in the communication between the biosynthetic and early endocytic pathways. It shares 81% identity with RAB31.

Publications

Species	Application	Title
human	WB	Nat Cell Biol; An ARF6-Exportin-5 axis delivers pre-miRNA cargo to tumour microvesicles.; Authors - James W Clancy
human	WB,IHC	Clin Cancer Res; The Immune-microenvironment Confers Chemoresistance of Colorectal Cancer through Macrophage-derived IL-6.; Authors - Yuan Yin
human	WB,IF	EMBO Rep; Rab22A recruits BLOC-1 and BLOC-2 to promote the biogenesis of recycling endosomes.; Authors - Saurabh Shakya ; KD Validated
human	IF	J Cell Sci; A specific subset of RabGTPases controls cell surface exposure of MT1-MMP, extracellular matrix degradation and three-dimensional invasion of macrophages.; Authors - Wiesner Christiane C
mouse	WB	Traffic; Rab GTPases regulating phagosome maturation are differentially recruited to mycobacterial phagosomes.; Authors - Seto Shintaro S
hamster, mouse, human	WB,IF	Mol Biol Cell; Rabex-5 is a Rab22 effector and mediates a Rab22-Rab5 signaling cascade in endocytosis.; Authors - Zhu Huaiping H ; KD Validated

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