Anticorps Monoclonal anti-Rab23

• Phare
• Validé par KD/KO

Rab23 Monoclonal Antibody for WB,ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

WB,ELISA

Conjugaison

Non conjugué

Publications

1

CloneNo.

3E12B6

N° de cat : 60056-1-Ig

Synonymes

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Galerie de données de validation

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  WB analysis of human brain using 60056-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 60056-1-Ig (RAB23 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 60056-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 60056-1-Ig (RAB23 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 60056-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 60056-1-Ig (RAB23 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: tissu cérébral humain, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below

Informations sur le produit

60056-1-Ig cible Rab23 dans les applications de WB,ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Rab23 Protéine recombinante Ag1588
• Nom complet: RAB23, member RAS oncogene family
• Masse moléculaire calculée: 237 aa, 27 kDa
• Poids moléculaire observé: 27-30 kDa
• Numéro d’acquisition GenBank: BC015021
• Symbole du gène: RAB23
• Identification du gène (NCBI): 51715
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Rab23 is a novel member of large Rab small GTPase family. It plays important roles during embryonic development. The brain-enriched Rab23 is the only Rab protein that is known to have a distinct function during central nervous system development, playing an essential role as a negative regulator of the Sonic Hedgehog (Shh) pathway. RAB23 has recently been identified in mesangial cells (MCs), and could serve as a biomarker that indicates the severity of FSGS ( focal segmental glomerulosclerosis). Mutations in RAB23 may result in ACPS2 ( acrocephalopolysyndactyly type 2).

Publications

Species	Application	Title
human,mouse	WB	Curr Biol; Planar Cell Polarity Effector Proteins Inturned and Fuzzy Form a Rab23 GEF Complex.; Authors - Andreas Gerondopoulos ; KD Validated