Anticorps Polyclonal de lapin anti-RAB27A

• Phare
• Validé par KD/KO

RAB27A Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (4)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

51

N° de cat : 17817-1-AP

Synonymes

GS2, GTP binding protein Ram, HsT18676, Rab 27, RAB27, RAB27A, RAM, Ras related protein Rab 27A

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Galerie de données de validation

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  WB analysis using 17817-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17817-1-AP (RAB27A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K562 using 17817-1-AP

  WB result of RAB27A antibody (17817-1-AP, 1:3000) with si-Control and si-RAB7B transfected K562 cells.

• 

  WB analysis of Apoptosised HeLa using 17817-1-AP

  Apoptosised HeLa cells were subjected to SDS PAGE followed by western blot with 17817-1-AP (RAB27A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 17817-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 17817-1-AP (RAB27A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 17817-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 17817-1-AP (RAB27A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 17817-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17817-1-AP (RAB27A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 17817-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 17817-1-AP (RAB27A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 17817-1-AP

  IP result of anti-RAB27A (IP:17817-1-AP, 4ug; Detection:17817-1-AP 1:1000) with K-562 cells lysate 2760 ug.

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  IHC staining of human malignant melanoma using 17817-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 17817-1-AP (RAB27A antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human malignant melanoma using 17817-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 17817-1-AP (RAB27A antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human stomach using 17817-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 17817-1-AP (RAB27A Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human stomach using 17817-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 17817-1-AP (RAB27A Antibody) at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HL-60, cellules HeLa apoptosées, cellules Jurkat, cellules K-562, cellules L02, tissu hépatique humain
• Résultats positifs en IP: cellules K-562,
• Résultats positifs en IHC: tissu de mélanome malin humain, tissu d'estomac humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 11 publications below
• WB: See 47 publications below
• IHC: See 2 publications below
• IF: See 5 publications below

Informations sur le produit

17817-1-AP cible RAB27A dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, canin, Humain, porc, poulet
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RAB27A Protéine recombinante Ag12136
• Nom complet: RAB27A, member RAS oncogene family
• Masse moléculaire calculée: 221 aa, 25 kDa
• Poids moléculaire observé: 25-27 kDa
• Numéro d’acquisition GenBank: BC107680
• Symbole du gène: RAB27A
• Identification du gène (NCBI): 5873
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Rab27A is a member of a large family of Ras-related small GTPases. The protein is membrane-bound and may be involved in protein transport and small GTPase mediated signal transduction. Recent studies suggest that Rab27A is associated with melanosomes in pigmented cells and regulates melanosome transport via its interaction with actin-based cellular motors such as myosin Va and myosin VIIa. RAB27A is also required for regulated secretion in cytotoxic T lymphocytes. Rab27A is implicated in several diseases, including Griscelli syndrome, choroideremia, and the Hermansky-Pudlak syndrome mouse model, gunmetal.

Publications

Species	Application	Title
human	WB	Nat Immunol; Exosomes mediate the cell-to-cell transmission of IFN-α-induced antiviral activity.; Authors - Li Jianhua J
human	WB	Adv Sci (Weinh); Exosome Release Is Regulated by mTORC1.; Authors - Wenchong Zou
human	WB	J Allergy Clin Immunol; A RAB27A 5' untranslated region structural variant associated with late-onset hemophagocytic lymphohistiocytosis and normal pigmentation.; Authors - Bianca Tesi
human	WB	Genes Dev; Cdx2 regulates endo-lysosomal function and epithelial cell polarity.; Authors - Gao Nan N
human	WB	Nat Commun; Exosomes maintain cellular homeostasis by excreting harmful DNA from cells.; Authors - Akiko Takahashi ; KD Validated
		Blood; Extracellular vesicles released by CD40/IL-4 stimulated chronic lymphocytic leukemia cells confer altered functional properties to CD4+ T cells.; Authors - Dawn T Smallwood

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