Anticorps Polyclonal de lapin anti-RAB27B

• Phare
• Validé par KD/KO

RAB27B Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

39

Avis (1)

N° de cat : 13412-1-AP

Synonymes

C25KG, RAB27B, Ras related protein Rab 27B

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Galerie de données de validation

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  WB analysis of rat spleen using 13412-1-AP

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 13412-1-AP (RAB27B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 13412-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 13412-1-AP (RAB27B antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 13412-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 13412-1-AP (RAB27B antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 13412-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 13412-1-AP (RAB27B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of DU 145 using 13412-1-AP

  DU 145 cells were subjected to SDS PAGE followed by western blot with 13412-1-AP (RAB27B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas cancer using 13412-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 13412-1-AP (RAB27B Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human pancreas cancer using 13412-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 13412-1-AP (RAB27B Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human oesophagus cancer using 13412-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 13412-1-AP (RAB27B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 13412-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 13412-1-AP (RAB27B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 13412-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13412-1-AP (RAB27B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 13412-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13412-1-AP (RAB27B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 13412-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 13412-1-AP (RAB27B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 13412-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 13412-1-AP (RAB27B antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 13412-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13412-1-AP (RAB27B Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human breast cancer using 13412-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13412-1-AP (RAB27B Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human colon cancer using 13412-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13412-1-AP (RAB27B Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human prostate cancer using 13412-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 13412-1-AP (RAB27B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate cancer using 13412-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 13412-1-AP (RAB27B antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human prostate cancer using 13412-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 13412-1-AP (RAB27B antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver cancer using 13412-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 13412-1-AP (RAB27B antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver cancer using 13412-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 13412-1-AP (RAB27B antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human prostate cancer using 13412-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 13412-1-AP (RAB27B antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human colon cancer using 13412-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13412-1-AP (RAB27B Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human testis using 13412-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 13412-1-AP (RAB27B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 13412-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 13412-1-AP (RAB27B antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 13412-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 13412-1-AP (RAB27B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of U-87 MG using 13412-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-87 MG cells using RAB27B antibody (13412-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: tissu splénique de rat, cellules A375, cellules DU 145, cellules MCF-7, cellules PC-3
• Résultats positifs en IHC: tissu de cancer du pancréas humain, tissu de cancer de la prostate humain, tissu de cancer de l'œsophage humain, tissu de cancer du côlon humain, tissu de cancer du foie humain, tissu de cancer du sein humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules MG U-87, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 10 publications below
• WB: See 32 publications below
• IHC: See 13 publications below
• IF: See 7 publications below

Informations sur le produit

13412-1-AP cible RAB27B dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, canin, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RAB27B Protéine recombinante Ag4064
• Nom complet: RAB27B, member RAS oncogene family
• Masse moléculaire calculée: 218 aa, 25 kDa
• Poids moléculaire observé: 25-30 kDa
• Numéro d’acquisition GenBank: BC027474
• Symbole du gène: RAB27B
• Identification du gène (NCBI): 5874
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The Rab27 GTPase subfamily consists of two closely related homologs, Rab27a and Rab27b. Northern blot analysis revealed that Rab27b is expressed significantly only in testis, in which the predominant transcript was 1.4 kb in size. An 8-kb mRNA of unknown origin was detected in many tissues. Rab27b is associated with tubulovesicle membranes in the parietal cell and Rab27b may play a role in stimulation-associated membrane recruitment and gastric acid secretion. Rab27b is recently reported as a marker for breast cancer progression. It regulates invasive growth and metastasis in ER-positive breast cancer cell lines, and increased expression is associated with poor prognosis in humans.

Publications

Species	Application	Title
mouse	IHC	Nature; Microenvironment-induced PTEN loss by exosomal microRNA primes brain metastasis outgrowth.; Authors - Lin Zhang
human	WB, IHC	Cell; Exosome transfer from stromal to breast cancer cells regulates therapy resistance pathways.; Authors - Mirjam C Boelens
human	WB	Immunity; MLKL, the Protein that Mediates Necroptosis, Also Regulates Endosomal Trafficking and Extracellular Vesicle Generation.; Authors - Seongmin Yoon
human	WB	Adv Sci (Weinh); Exosome Release Is Regulated by mTORC1.; Authors - Wenchong Zou
mouse	IF	Nat Commun; Identification of genes associated with cortical malformation using a transposon-mediated somatic mutagenesis screen in mice.; Authors - I-Ling Lu
human	WB	Nat Commun; Exosomes maintain cellular homeostasis by excreting harmful DNA from cells.; Authors - Akiko Takahashi

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Kacie (Verified Customer) (09-12-2022)

Antibody works well for both ICC and WB.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: M17 Cells

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