Anticorps Monoclonal anti-RAB27B

• Phare
• Validé par KD/KO

RAB27B Monoclonal Antibody for IF/ICC, FC (Intra)

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

IF/ICC, FC (Intra)

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2B9G9

N° de cat : CL488-66944

Synonymes

C25KG, Ras-related protein Rab-27B, Ras related protein Rab 27B, EC:3.6.5.2, 2B9G9

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Galerie de données de validation

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  IF Staining of A431 using CL488-66944

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CoraLite® Plus 488 RAB27B antibody (CL488-66944, Clone: 2B9G9 ) at dilution of 1:200, CL594-Phalloidin (red).

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  FC experiment of A431 using CL488-66944

  1x10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated RAB27B Monoclonal antibody (CL488-66944, Clone:2B9G9)(red), or 0.4 ug Isotype Control (CL488-66360-2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en IF/ICC: cellules A431,
• Résultats positifs en FC (Intra): cellules A431,

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-66944 cible RAB27B dans les applications de IF/ICC, FC (Intra) et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: RAB27B Protéine recombinante Ag18756
• Nom complet: RAB27B, member RAS oncogene family
• Masse moléculaire calculée: 218 aa, 25 kDa
• Poids moléculaire observé: 25 kDa
• Numéro d’acquisition GenBank: BC027474
• Symbole du gène: RAB27B
• Identification du gène (NCBI): 5874
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The Rab27 GTPase subfamily consists of two closely related homologs, Rab27a and Rab27b. Northern blot analysis revealed that Rab27b is expressed significantly only in testis, in which the predominant transcript was 1.4 kb in size. An 8-kb mRNA of unknown origin was detected in many tissues. Rab27b is associated with tubulovesicle membranes in the parietal cell and Rab27b may play a role in stimulation-associated membrane recruitment and gastric acid secretion. Rab27b is recently reported as a marker for breast cancer progression. It regulates invasive growth and metastasis in ER-positive breast cancer cell lines, and increased expression is associated with poor prognosis in humans.

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