Anticorps Polyclonal de lapin anti-RAB39

• Phare
• Validé par KD/KO

RAB39 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IP, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications(4)

N° de cat : 13355-1-AP

Synonymes

Rab 39, RAB39, RAB39A, Ras related protein Rab 39A

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Galerie de données de validation

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  WB analysis of mouse brain using 13355-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13355-1-AP (RAB39 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 13355-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13355-1-AP (RAB39 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 13355-1-AP

  IP result of anti-RAB39 (IP:13355-1-AP, 4ug; Detection:13355-1-AP 1:500) with mouse brain tissue lysate 4000ug.

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  IHC staining of human gliomas using 13355-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13355-1-AP (RAB39 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 13355-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13355-1-AP (RAB39 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 13355-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 13355-1-AP (RAB39 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 13355-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 13355-1-AP (RAB39 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human gliomas using 13355-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 13355-1-AP (RAB39 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human gliomas using 13355-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 13355-1-AP (RAB39 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 13355-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 13355-1-AP (RAB39 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human brain using 13355-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 13355-1-AP (RAB39 Antibody) at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral humain
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu de gliome humain, tissu cérébral humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

13355-1-AP cible RAB39 dans les applications de WB, IP, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: canin, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RAB39 Protéine recombinante Ag4151
• Nom complet: RAB39, member RAS oncogene family
• Masse moléculaire calculée: 217 aa, 25 kDa
• Poids moléculaire observé: 25 kDa
• Numéro d’acquisition GenBank: BC028064
• Symbole du gène: RAB39
• Identification du gène (NCBI): 54734
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Nat Commun; C9orf72-catalyzed GTP loading of Rab39A enables HOPS-mediated membrane tethering and fusion in mammalian autophagy; Authors - Shen Zhang ; KD Validated
human	WB	Oncotarget; Prominent role of RAB39A-RXRB axis in cancer development and stemness.; Authors - Tokuhiro Chano
human	IF	J Biochem; RAB39A: a Rab small GTPase with a prominent role in cancer stemness.; Authors - Tokuhiro Chano
canine	WB,IP	Cell Rep; Rab39 and its effector UACA regulate basolateral exosome release from polarized epithelial cells.; Authors - Takahide Matsui

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