Anticorps Polyclonal de lapin anti-RAB39B

• Phare
• Validé par KD/KO

RAB39B Polyclonal Antibody for IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

10

Avis (1)

N° de cat : 12162-1-AP

Synonymes

RAB39B, Ras related protein Rab 39B

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Galerie de données de validation

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  WB analysis of fetal human brain using 12162-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 12162-1-AP (RAB39B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Y79 using 12162-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 12162-1-AP (RAB39B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Y79 using 12162-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 12162-1-AP (RAB39B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 12162-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12162-1-AP (RAB39B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 12162-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12162-1-AP (RAB39B antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  IP experiment of SH-SY5Y using 12162-1-AP

  IP result of anti-RAB39B (IP:12162-1-AP, 4ug; Detection:12162-1-AP 1:1000) with SH-SY5Y cells lysate 2200 ug.

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  IP experiment of mouse brain using 12162-1-AP

  IP Result of anti-RAB39B (IP:12162-1-AP, 4ug; Detection:12162-1-AP 1:300) with mouse brain tissue lysate 4000ug.

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  IHC staining of human lymphoma using 12162-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 12162-1-AP (RAB39B antibody) at dilution of 1:50 (under 10x lens).

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Applications testées

• Résultats positifs en WB: tissu cérébral humain fœtal, cellules Y79, tissu cérébral de souris, tissu cérébral humain
• Résultats positifs en IP: cellules SH-SY5Y, tissu cérébral de souris
• Résultats positifs en IHC: tissu de lymphome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IF: See 4 publications below
• IP: See 1 publications below

Informations sur le produit

12162-1-AP cible RAB39B dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RAB39B Protéine recombinante Ag2803
• Nom complet: RAB39B, member RAS oncogene family
• Masse moléculaire calculée: 213 aa, 25 kDa
• Poids moléculaire observé: 25 kDa
• Numéro d’acquisition GenBank: BC009714
• Symbole du gène: RAB39B
• Identification du gène (NCBI): 116442
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Nat Commun; C9orf72-catalyzed GTP loading of Rab39A enables HOPS-mediated membrane tethering and fusion in mammalian autophagy; Authors - Shen Zhang ; KO Validated
mouse	WB	Am J Hum Genet; Mutations in RAB39B Cause X-Linked Intellectual Disability and Early-Onset Parkinson Disease with α-Synuclein Pathology.; Authors - Gabrielle R Wilson
human	IP	EMBO J; Loss of C9ORF72 impairs autophagy and synergizes with polyQ Ataxin-2 to induce motor neuron dysfunction and cell death.; Authors - Chantal Sellier
human	WB	Mov Disord; Clinical and Neuropathological Features Associated With Loss of RAB39B.; Authors - Yujing Gao
mouse	IF,WB	Acta Neuropathol Commun; Synaptic localization of C9orf72 regulates post-synaptic glutamate receptor 1 levels.; Authors - Shangxi Xiao
human	WB,IF	Brain Pathol; RAB39B is redistributed in dementia with Lewy bodies and is sequestered within aβ plaques and Lewy bodies.; Authors - David J Koss

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

David (Verified Customer) (09-25-2019)

The antibodiy works well in membrane enriched fractionates generated from human brain homogenate. Note that several higher molecular weight bands are seen when using crude lysates generated from the same samples.Image details: lane 1 = molecular weight ladder, lanes 2-7 = membrane enriched fractionates (2µg/lane).Membrane blocked in 5% milk powder containing TBST overnight at 4oC. Primary antibody: RAB39B (1:1000) in 5% BSA containing TBST, 1hr incubation at RT. Secondar antibody: y Goat anti Rabbit-HRP (1:5000, 1hr RT) in 5% milk powder containing TBST.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human brain tissue

RAB39B Antibody Western Blot, validation (1:1000 dilution) in Human brain tissue (Cat no:12162-1-AP)