Anticorps Polyclonal de lapin anti-RAB3B/C

RAB3B/C Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications(5)

N° de cat : 15774-1-AP

Synonymes

RAB3B, Ras related protein Rab 3B, Ras-related protein Rab-3B

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Galerie de données de validation

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  WB analysis using 15774-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15774-1-AP (RAB3B/C antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 15774-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15774-1-AP (RAB3B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15774-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15774-1-AP (RAB3B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of U-251 using 15774-1-AP

  U-251 cells were subjected to SDS PAGE followed by western blot with 15774-1-AP (RAB3B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 15774-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15774-1-AP (RAB3B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 15774-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15774-1-AP (RAB3B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 15774-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 15774-1-AP (RAB3B antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 15774-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 15774-1-AP (RAB3B antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human pancreas using 15774-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 15774-1-AP (RAB3B Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas using 15774-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 15774-1-AP (RAB3B Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 15774-1-AP

  Immunofluorescent analysis of HeLa cells using 15774-1-AP (RAB3B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HepG2 using 15774-1-AP

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug RAB3B/C Polyclonal antibody (15774-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules U-251, tissu cérébral de rat, tissu cérébral humain
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu pancréatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa
• Résultats positifs en FC (Intra): cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

15774-1-AP cible RAB3B/C dans les applications de WB, IHC, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RAB3B/C Protéine recombinante Ag8441
• Nom complet: RAB3B, member RAS oncogene family
• Masse moléculaire calculée: 219 aa, 25 kDa
• Poids moléculaire observé: 25 kDa
• Numéro d’acquisition GenBank: BC005035
• Symbole du gène: RAB3B
• Identification du gène (NCBI): 5865
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Ann Neurol; PMID: 32167609; Authors - Jimena Monzón-Sandoval
mouse	WB	Hum Mol Genet; Progressive axonal transport and synaptic protein changes correlate with behavioral and neuropathological abnormalities in the heterozygous Q175 KI mouse model of Huntington's disease.; Authors - Gaynor A Smith
human	WB,IF	Cell Death Dis; Long noncoding RNA ENST00000436340 promotes podocyte injury in diabetic kidney disease by facilitating the association of PTBP1 with RAB3B; Authors - Jinxiu Hu
human	WB	Adv Sci (Weinh); Evoked Weibel-Palade Body Exocytosis Modifies the Endothelial Cell Surface by Releasing a Substrate-Selective Phosphodiesterase; Authors - Johannes Naß
human	IHC	Sci Rep; Pan-cancer analysis reveals correlation between RAB3B expression and tumor heterogeneity, immune microenvironment, and prognosis in multiple cancers; Authors - Xu-Sheng Liu

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