Anticorps Polyclonal de lapin anti-RAB3IP

• Phare
• Validé par KD/KO

RAB3IP Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

16

Avis (2)

N° de cat : 12321-1-AP

Synonymes

Rab 3A interacting protein, Rab3A interacting protein, RAB3IP, RAB3IP/Rabin8, Rabin 3, RABIN3, Rabin8, SSX2 interacting protein

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Galerie de données de validation

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  WB analysis of human brain using 12321-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12321-1-AP (RAB3IP/Rabin8 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12321-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12321-1-AP (RAB3IP antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 12321-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 12321-1-AP (RAB3IP/Rabin8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 12321-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12321-1-AP (RAB3IP/Rabin8 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 12321-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 12321-1-AP (RAB3IP/Rabin8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse lung using 12321-1-AP

  IP Result of anti-RAB3IP/Rabin8 (IP:12321-1-AP, 3ug; Detection:12321-1-AP 1:1000) with mouse lung tissue lysate 4500ug.

• 

  IHC staining of human stomach cancer using 12321-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12321-1-AP (RAB3IP/Rabin8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 12321-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12321-1-AP (RAB3IP/Rabin8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 12321-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RAB3IP/Rabin8 antibody (12321-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cardiaque de souris, tissu cardiaque humain, tissu cérébral de rat, tissu cérébral humain, tissu pulmonaire de souris
• Résultats positifs en IP: tissu pulmonaire de souris
• Résultats positifs en IHC: tissu de cancer de l'estomac humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 6 publications below
• WB: See 15 publications below
• IF: See 6 publications below
• IP: See 3 publications below

Informations sur le produit

12321-1-AP cible RAB3IP dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, canin, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RAB3IP Protéine recombinante Ag2974
• Nom complet: RAB3A interacting protein (rabin3)
• Masse moléculaire calculée: 476 aa, 53 kDa
• Poids moléculaire observé: 53/51/43 kDa
• Numéro d’acquisition GenBank: BC015548
• Symbole du gène: RAB3IP
• Identification du gène (NCBI): 117177
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RAB3IP, also hnown as Rabin8, is a deduced 476-amino acid protein containing a central coiled-coil domain, a bipartite nuclear localization signal, and a C-terminal endoplasmic reticulum retention motif. Rabin8 has several sites for N-glycosylation and phosphorylation. Rabin8 plays important roles in various cellular processes, including ciliogenesis.The GEF activity of Rabin8 towards Rab8 is essential for ciliogenesis and cyst apical membrane transport,but not for exocytosis of discoidal/fusiform-shaped vesicles. This antibody can recognize isoforms with predicted MW's of 53 kDa, 51 kDa, 43 kDa, 45 kDa, 33 kDa and 35 kDa, 29 kDa respectively.

Publications

Species	Application	Title
canine	WB,IF	Nat Cell Biol; A molecular network for de novo generation of the apical surface and lumen.; Authors - Bryant David M DM ; KD Validated
rat	WB, IF	Neuron; Chemical genetic identification of NDR1/2 kinase substrates AAK1 and Rabin8 Uncovers their roles in dendrite arborization and spine development.; Authors - Sila K Ultanir
mouse	WB, IF	PLoS Biol; GSK3β-Dzip1-Rab8 Cascade Regulates Ciliogenesis after Mitosis.; Authors - Boyan Zhang
human	WB	J Cell Biol; Chibby promotes ciliary vesicle formation and basal body docking during airway cell differentiation.; Authors - Michael C Burke
human	WB	EMBO J; Phosphoproteomic screening identifies Rab GTPases as novel downstream targets of PINK1.; Authors - Yu-Chiang Lai
human	WB	EMBO J; Insufficiency of ciliary cholesterol in hereditary Zellweger syndrome.; Authors - Tatsuo Miyamoto ; KO Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Silvia (Verified Customer) (09-30-2021)

Immunofluorescence (1:200) works well on NPC

• Applications: Immunofluorescence

FH

Sara (Verified Customer) (04-07-2020)

I got a quite similar staining to the one shown in the example figure of this product data sheet. Not very sharp staining (a bit all over the cell), but working. Next I will try in WB.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Spleen / B cells