Anticorps Monoclonal anti-RAB43

RAB43 Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain et plus (1)

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

3B2A8

Avis (3)

N° de cat : 66654-1-Ig

Synonymes

ISY1, RAB11B, RAB41, Ras related protein Rab 41, Ras related protein Rab 43

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Galerie de données de validation

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  WB analysis of human brain using 66654-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 66654-1-Ig (RAB43 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66654-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66654-1-Ig (RAB43 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 66654-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66654-1-Ig (RAB43 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules HEK-293, cellules HepG2, cellules Jurkat, cellules L02, tissu cérébral humain, tissu placentaire humain

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

66654-1-Ig cible RAB43 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: RAB43 Protéine recombinante Ag26911
• Nom complet: RAB43, member RAS oncogene family
• Masse moléculaire calculée: 212 aa, 23 kDa
• Poids moléculaire observé: 23 kDa
• Numéro d’acquisition GenBank: BC062319
• Symbole du gène: RAB43
• Identification du gène (NCBI): 339122
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Ras-related GTP-binding protein 43 (RAB43) is a member of the Ras superfamily. RAB43 is mainly located in endoplasmic reticulum and Golgi, and plays a role as a key regulator of vesicle movement, signal transduction and tethering membrane events in membrane trafficking.

Publications

Species	Application	Title
mouse,human	WB	J Cell Sci; ARF6 plays a general role in targeting palmitoylated proteins from the Golgi to the plasma membrane; Authors - Juan Wang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Aleksandr (Verified Customer) (07-19-2025)

Antibody recognise a specific band of human Rab43. Overnight incubation at 4C

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: RKO

RAB43 Antibody Western Blot validation (1:2000 dilution) in RKO (Cat no:66654-1-Ig)

FH

Tsimafei (Verified Customer) (11-19-2024)

Great antibody for Rab43 detection

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: U2OS

RAB43 Antibody Western Blot validation (1:2000 dilution) in U2OS (Cat no:66654-1-Ig)

FH

Moritz (Verified Customer) (09-12-2024)

Several bands were observable in WB. HEK293T Wt lysate.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: HEK293T

RAB43 Antibody Western Blot validation (1:2000 dilution) in HEK293T (Cat no:66654-1-Ig)

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