Anticorps Polyclonal de lapin anti-RAB5B

• Phare
• Validé par KD/KO

RAB5B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(5)

Avis (1)

N° de cat : 27403-1-AP

Synonymes

EC:3.6.5.2, Ras related protein Rab 5B, Ras-related protein Rab-5B

---

Galerie de données de validation

• 

  WB analysis of HeLa using 27403-1-AP

  WB result of RAB5B antibody (27403-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAB5B transfected HeLa cells.

• 

  WB analysis of mouse lung using 27403-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 27403-1-AP (RAB5B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 27403-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 27403-1-AP (RAB5B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 27403-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 27403-1-AP (RAB5B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 27403-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 27403-1-AP (RAB5B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 27403-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 27403-1-AP (RAB5B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 27403-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 27403-1-AP (RAB5B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon using 27403-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27403-1-AP (RAB5B antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).

• 

  IHC staining of human stomach using 27403-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 27403-1-AP (RAB5B antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 27403-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RAB5B antibody (27403-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

---

Applications testées

• Résultats positifs en WB: tissu pulmonaire de souris, cellules HEK-293, cellules HeLa, cellules HepG2, cellules Jurkat, cellules NIH/3T3
• Résultats positifs en IHC: tissu d'estomac humain, tissu de côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 1 publications below

Informations sur le produit

27403-1-AP cible RAB5B dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RAB5B Protéine recombinante Ag26584
• Nom complet: RAB5B, member RAS oncogene family
• Masse moléculaire calculée: 215 aa, 24 kDa
• Poids moléculaire observé: 27 kDa
• Numéro d’acquisition GenBank: BC032740
• Symbole du gène: RAB5B
• Identification du gène (NCBI): 5869
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RAB5B (Ras-related protein Rab-5B) belongs to the Rab family. Rab5 is specifically localized to the early compartment and regulates endocytosis (PMID:1516130, PMID:9087439). There are three isoforms of Rab5 namely, Rab5a, Rab5b, and Rab5c present in EE of mammalian cells (PMID:7789520). Recently, we have shown that Rab5a regulates fluid-phase endocytosis whereas receptor-mediated endocytosis is regulated by Rab5b in Leishmania (PMID:27226564).

Publications

Species	Application	Title
mouse	WB	bioRxiv; The Sde Phosphoribosyl-Linked Ubiquitin Transferases protect the Legionella pneumophila vacuole from degradation by the host; Authors - Seongok Kim ; KD Validated
mouse	WB	Proc Natl Acad Sci U S A; The Sde phosphoribosyl-linked ubiquitin transferases protect the Legionella pneumophila vacuole from degradation by the host; Authors - Seongok Kim
mouse,human	WB,IHC	Mol Hum Reprod; Hsa_circRNA_BECN1 acts as a ceRNA to promote polycystic ovary syndrome progression by sponging the miR-619-5p/Rab5b axis; Authors - Hairui Fan
human	WB	Mol Metab; Tetraspanin7 in Adipose Tissue Remodeling and Its Impact on Metabolic Health; Authors - Shino Nemoto
mouse	WB	Development; A crucial role for Arf6 in the response of commissural axons to Slit.; Authors - Mariko Kinoshita-Kawada

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Xin (Verified Customer) (04-15-2022)

It is ok to detect endogenous Rab5B, although the signal is a little weak.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mouse cortex lysate

RAB5B Antibody Western Blot validation (1:1000 dilution) in mouse cortex lysate (Cat no:27403-1-AP)

{{ptg:RelatedPrimaryAntibodies}}