Anticorps Polyclonal de lapin anti-RAB8A

• Phare
• Validé par KD/KO

RAB8A Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IHC, IF/ICC, IP, RIP, ELISA

Conjugaison

Non conjugué

Publications(32)

Avis (1)

N° de cat : 55296-1-AP

Synonymes

MEL, Oncogene c mel, RAB8, RAB8A, Ras related protein Rab 8A

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Galerie de données de validation

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  WB analysis using 55296-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55296-1-AP (RAB8A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 55296-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 55296-1-AP (RAB8A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 55296-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 55296-1-AP (RAB8A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 55296-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 55296-1-AP (RAB8A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 55296-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 55296-1-AP (RAB8A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skin using 55296-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 55296-1-AP (RAB8A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 55296-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 55296-1-AP (RAB8A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 55296-1-AP

  IP result of anti-RAB8A (IP:55296-1-AP, 4ug; Detection:55296-1-AP 1:600) with HeLa cells lysate 1320 ug.

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  IHC staining of human colon cancer using 55296-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55296-1-AP (RAB8A Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon cancer using 55296-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55296-1-AP (RAB8A Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of NIH/3T3 using 55296-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using RAB8A antibody (55296-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HeLa, cellules HepG2, cellules NIH/3T3, tissu cérébral humain, tissu cutané de souris, tissu pulmonaire de souris
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules NIH/3T3,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 21 publications below
• IHC: See 2 publications below
• IF: See 14 publications below
• IP: See 2 publications below
• RIP: See 1 publications below

Informations sur le produit

55296-1-AP cible RAB8A dans les applications de WB, IHC, IF/ICC, IP, RIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, canin, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: RAB8A, member RAS oncogene family
• Masse moléculaire calculée: 24 kDa
• Poids moléculaire observé: 24 kDa
• Numéro d’acquisition GenBank: NM_005370
• Symbole du gène: RAB8A
• Identification du gène (NCBI): 4218
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The Rab8 GTPase is a member of the Ras superfamily that functions in protein transport and membrane restructuring. RAB8 has two isoforms, RAB8A and RAB8B, that share 40% amino acid identity in the C-terminal variable region. RAB8 activity is regulated by specific guanine nucleotide exchange factors (GEFs), that mediate GTP loading, and GTPase activating proteins (GAPs) that convert them into the inactive GDP-bound form. RAB8 participates in recycling of the α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors to the dendritic spine surface as well as in the intracellular transport of the metabotropic glutamate receptor type 1 in neuronal cells. Rab8 is activated at the base of the cilium by its guanine nucleotide exchanger Rabin8. The antibody 11792-1-AP can detect both RAB8A and RAB8B. 55295-1-AP is specific to RAB8B and 55296-1-AP is specific to RAB8A.

Publications

Species	Application	Title
canine	WB,IF	Nat Cell Biol; A molecular network for de novo generation of the apical surface and lumen.; Authors - Bryant David M DM ; KD Validated
mouse	WB,IF	Cell Res; NudCL2 is an autophagy receptor that mediates selective autophagic degradation of CP110 at mother centrioles to promote ciliogenesis.; Authors - Min Liu
human	RIP	Adv Sci (Weinh); Primary Cilia Formation Mediated by Hsa_Circ_0005185/OTUB1/RAB8A Complex Inhibits Prostate Cancer Progression by Suppressing Hedgehog Signaling Pathway; Authors - Aoyu Fan
mouse	IF	Circ Res; Rasip1-Mediated Rho GTPase Signaling Regulates Blood Vessel Tubulogenesis via Nonmuscle Myosin II.; Authors - David M Barry
human	WB, IF	J Cell Biol; Chibby promotes ciliary vesicle formation and basal body docking during airway cell differentiation.; Authors - Michael C Burke
mouse	IF	Nat Commun; Ciliopathy-associated gene Cc2d2a promotes assembly of subdistal appendages on the mother centriole during cilia biogenesis.; Authors - Shobi Veleri

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Sara (Verified Customer) (04-30-2020)

Unfortunately this antibody did not work for my samples. I followed all the instructions in the "WB protocol for Rab8a antibody 55296-1-AP" that Proteintech provides. I did not get almost any signal when following those instructions (primary antibody 1:600 for 1h at 30-35°C). After repeating the experiment incubating the membrane with 1:600 primary antibody O/N at 4°C I got better signal, but still couldn't see a band of 24 kDa.

• Applications: Western Blot
• Primary Antibody Dilution: 1:600
• Cell Tissue Type: Primary B cells from mouse spleen

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