Anticorps Polyclonal de lapin anti-RAB8B

RAB8B Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

N° de cat : 55295-1-AP

Synonymes

RAB8, Ras related protein Rab 8B, Ras-related protein Rab-8B

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Galerie de données de validation

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  WB analysis of mouse skin using 55295-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 55295-1-AP (RAB8B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 55295-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55295-1-AP (RAB8B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 55295-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55295-1-AP (RAB8B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 55295-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 55295-1-AP (RAB8B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 55295-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 55295-1-AP (RAB8B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: tissu cutané de souris, tissu cérébral humain, tissu cutané de rat, tissu de côlon de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

55295-1-AP cible RAB8B dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: RAB8B, member RAS oncogene family
• Masse moléculaire calculée: 24 kDa
• Poids moléculaire observé: 28 kDa
• Numéro d’acquisition GenBank: NM_016530
• Symbole du gène: RAB8B
• Identification du gène (NCBI): 51762
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The Rab8 GTPase is a member of the Ras superfamily that functions in protein transport and membrane restructuring. RAB8 has two isoforms, RAB8A and RAB8B, that share 40% amino acid identity in the C-terminal variable region. RAB8 activity is regulated by specific guanine nucleotide exchange factors (GEFs), that mediate GTP loading, and GTPase activating proteins (GAPs) that convert them into the inactive GDP-bound form. RAB8 participates in recycling of the α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors to the dendritic spine surface as well as in the intracellular transport of the metabotropic glutamate receptor type 1 in neuronal cells. Rab8 is activated at the base of the cilium by its guanine nucleotide exchanger Rabin8. The antibody 11792-1-AP can detect both RAB8A and RAB8B. 55295-1-AP is specific to RAB8B and 55296-1-AP is specific to RAB8A.

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