Anticorps Polyclonal de lapin anti-RABEP2

RABEP2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 14625-1-AP

Synonymes

FRA, Rabaptin 5beta, RABEP2, RABPT5B

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Galerie de données de validation

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  WB analysis of Jurkat using 14625-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14625-1-AP (RABEP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 14625-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14625-1-AP (RABEP2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14625-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14625-1-AP (RABEP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 14625-1-AP

  IP Result of anti-RABEP2 (IP:14625-1-AP, 4ug; Detection:14625-1-AP 1:1000) with mouse liver tissue lysate 4000ug.

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  IHC staining of human cervical cancer using 14625-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14625-1-AP (RABEP2 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 14625-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14625-1-AP (RABEP2 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 14625-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14625-1-AP (RABEP2 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 14625-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14625-1-AP (RABEP2 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 14625-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using RABEP2 antibody (14625-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HeLa, tissu hépatique de souris
• Résultats positifs en IP: tissu hépatique de souris
• Résultats positifs en IHC: tissu de cancer du col de l'utérus humain, tissu de cancer de la prostate humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 7 publications below
• IF: See 2 publications below

Informations sur le produit

14625-1-AP cible RABEP2 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RABEP2 Protéine recombinante Ag6197
• Nom complet: rabaptin, RAB GTPase binding effector protein 2
• Masse moléculaire calculée: 64 kDa
• Poids moléculaire observé: 59-62 kDa
• Numéro d’acquisition GenBank: BC058900
• Symbole du gène: RABEP2
• Identification du gène (NCBI): 79874
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RABEP2, also named as RABPT5B, plays a role in membrane trafficking and in homotypic early endosome fusion. It has two isoforms with MW 59-63 kDa.

Publications

Species	Application	Title
human	WB	Am J Hum Genet; A cross-species approach using an in vivo evaluation platform in mice demonstrates that sequence variation in human RABEP2 modulates ischemic stroke outcomes; Authors - Han Kyu Lee
mouse	WB	Stroke; Variants of Rab GTPase-Effector Binding Protein-2 Cause Variation in the Collateral Circulation and Severity of Stroke.; Authors - Jennifer L Lucitti
human	WB,IF	Sci Rep; Rab-GTPase binding effector protein 2 (RABEP2) is a primed substrate for Glycogen Synthase kinase-3 (GSK3).; Authors - Lisa Logie
mouse	WB	J Mol Cell Cardiol; Decreased inspired oxygen stimulates de novo formation of coronary collaterals in adult heart.; Authors - Amir Aghajanian
mouse	WB	J Hematol Oncol; LncRNAs H19 and HULC, activated by oxidative stress, promote cell migration and invasion in cholangiocarcinoma through a ceRNA manner.; Authors - Wen-Tao Wang
mouse	WB,IF	Arterioscler Thromb Vasc Biol; Hypoxia-Preconditioned Bone Marrow Mesenchymal Stem Cells Improved Cerebral Collateral Circulation and Stroke Outcome in Mice; Authors - Hao Tian