Anticorps Polyclonal de lapin anti-RABL3

• Phare
• Validé par KD/KO

RABL3 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 11916-1-AP

Synonymes

Rab like protein 3, RABL3

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Galerie de données de validation

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  WB analysis using 11916-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11916-1-AP (RABL3 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of human lung using 11916-1-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 11916-1-AP (RABL3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 11916-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11916-1-AP (RABL3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HUVEC using 11916-1-PBS

  HUVEC cells were subjected to SDS PAGE followed by western blot with 11916-1-AP (RABL3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 11916-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11916-1-AP (RABL3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 11916-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11916-1-AP (RABL3 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse lung using 11916-1-AP

  IP Result of anti-RABL3 (IP:11916-1-AP, 3ug; Detection:11916-1-AP 1:300) with mouse lung tissue lysate 4000ug.

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  IHC staining of human breast cancer using 11916-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11916-1-AP (RABL3 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 11916-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11916-1-AP (RABL3 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver cancer using 11916-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11916-1-AP (RABL3 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 11916-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11916-1-AP (RABL3 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human breast cancer using 11916-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11916-1-AP (RABL3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 11916-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11916-1-AP (RABL3 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human cervical cancer using 11916-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 11916-1-AP (RABL3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human cervical cancer using 11916-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 11916-1-AP (RABL3 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 11916-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 11916-1-AP (RABL3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 11916-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 11916-1-AP (RABL3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 11916-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 11916-1-AP (RABL3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 11916-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 11916-1-AP (RABL3 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of A549 using 11916-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using RABL3 antibody (11916-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  FC experiment of HEK-293 using 11916-1-AP

  1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human RABL3 (11916-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HUVEC, cellules U-251, tissu cérébral de souris, tissu cérébral humain, tissu pulmonaire humain
• Résultats positifs en IP: tissu pulmonaire de souris
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer du col de l'utérus humain, tissu de cancer du foie humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules A549,
• Résultats positifs en cytométrie: cellules HEK-293,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:200-1:800
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 2 publications below

Informations sur le produit

11916-1-AP cible RABL3 dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RABL3 Protéine recombinante Ag2531
• Nom complet: RAB, member of RAS oncogene family-like 3
• Masse moléculaire calculée: 236 aa, 26 kDa
• Poids moléculaire observé: 26 kDa
• Numéro d’acquisition GenBank: BC020832
• Symbole du gène: RABL3
• Identification du gène (NCBI): 285282
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The gene of Rabl3 is located on 3q13.3 and is composed of eight exons and seven introns. It is a member of the Rab subfamily, the largest group in the Ras superfamily of small guanosine triphosphatases (GTPases).

Publications

Species	Application	Title
human	WB	Oncol Rep; Evaluation of the novel gene Rabl3 in the regulation of proliferation and motility in human cancer cells.; Authors - Li Qi Q ; KD Validated
human	WB	Med Sci Monit; High Expression of Rab-like 3 (Rabl3) is Associated with Poor Survival of Patients with Non-Small Cell Lung Cancer via Repression of MAPK8/9/10-Mediated Autophagy.; Authors - Weihua Zhang ; KD Validated