Anticorps Monoclonal anti-RAC1

• Phare
• Validé par KD/KO

RAC1 Monoclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, PLA, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

29

CloneNo.

4A4B11

N° de cat : 66122-1-Ig

Synonymes

MIG5, p21 Rac1, RAC1, RAC1b, Ras like protein TC25, TC 25, TC25

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Galerie de données de validation

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  WB analysis using 66122-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66122-1-Ig (Rac1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 66122-1-Ig

  WB result of RAC1 antibody (66122-1-Ig, 1:2000) with si-control and si-RAC1 transfected NIH3T3 cells.

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  WB analysis of human brain using 66122-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 66122-1-Ig (RAC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66122-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66122-1-Ig (Rac1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of HL-60 using 66122-1-Ig

  IP Result of anti-RAC1 (IP:66122-1-Ig, 3ug; Detection:66122-1-Ig 1:700) with HL-60 cells lysate 4000ug.

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  IHC staining of human colon cancer using 66122-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66122-1-Ig (RAC1 Antibody) at dilution of 1:400 (under 10x lens).

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  IHC staining of human colon cancer using 66122-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66122-1-Ig (RAC1 Antibody) at dilution of 1:400 (under 40x lens).

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  IF Staining of NIH/3T3 using 66122-1-Ig

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed NIH/3T3 cells using 66122-1-Ig(Rac1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of HeLa using 66122-1-Ig

  Immunofluorescent analysis of (10% Formaldehyde ) fixed HeLa cells using 66122-1-Ig(Rac1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules HL-60, cellules HSC-T6, cellules HUVEC, cellules NIH/3T3, cellules RAW 264.7, tissu cérébral humain
• Résultats positifs en IP: cellules HL-60, cellules HL-60, cellules HSC-T6, cellules HUVEC, cellules NIH/3T3, cellules RAW 264.7
• Résultats positifs en IHC: tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules NIH/3T3, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 25 publications below
• IHC: See 2 publications below
• IF: See 7 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

66122-1-Ig cible RAC1 dans les applications de WB, PLA, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: RAC1 Protéine recombinante Ag17779
• Nom complet: ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1)
• Masse moléculaire calculée: 21 kDa
• Poids moléculaire observé: 21 kDa
• Numéro d’acquisition GenBank: BC004247
• Symbole du gène: RAC1
• Identification du gène (NCBI): 5879
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RAC1b, also named as TC25, belongs to the small GTPase superfamily and Rho family. RAC1 is a plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, it binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles.

Publications

Species	Application	Title
mouse	WB	Mol Cell; TCR activation directly stimulates PYGB-dependent glycogenolysis to fuel the early recall response in CD8+ memory T cells.; Authors - Huafeng Zhang
human	PLA	Nat Commun; Regulation of local GTP availability controls RAC1 activity and cell invasion; Authors - Anna Bianchi-Smiraglia
mouse	WB	Nat Commun; TLR4 signalling via Piezo1 engages and enhances the macrophage mediated host response during bacterial infection.; Authors - Jing Geng
mouse	WB	Circ. Res.; Inhibition of KLF5-Myo9b-RhoA Pathway-Mediated Podosome Formation in Macrophages Ameliorates Abdominal Aortic Aneurysm.; Authors - Dong Ma
human	WB	Oncogene; Smoking-associated upregulation of CBX3 suppresses ARHGAP24 expression to activate Rac1 signaling and promote tumor progression in lung adenocarcinoma.; Authors - Xin Jin
human	WB	Cell Chem Biol; Phosphorylation of guanosine monophosphate reductase triggers a GTP-dependent switch from pro- to anti-oncogenic function of EPHA4.; Authors - David W Wolff

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