Anticorps Polyclonal de lapin anti-RAC1/2/3

• Phare
• Validé par KD/KO

RAC1/2/3 Polyclonal Antibody for IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, ELISA

Conjugaison

Non conjugué

Publications

8

N° de cat : 16117-1-AP

Synonymes

p21 Rac3, RAC1/2/3, RAC3

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Galerie de données de validation

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  WB analysis using 16117-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16117-1-AP (RAC1/2/3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16117-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16117-1-AP (RAC1/2/3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 16117-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16117-1-AP (RAC1/2/3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 16117-1-AP

  IP Result of anti-RAC1/2/3 (IP:16117-1-AP, 3ug; Detection:16117-1-AP 1:500) with mouse brain tissue lysate 2640ug.

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Applications testées

• Résultats positifs en WB: cellules SH-SY5Y, tissu cérébral de rat, tissu cérébral de souris, tissu cérébral humain
• Résultats positifs en IP: tissu cérébral de souris

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 8 publications below

Informations sur le produit

16117-1-AP cible RAC1/2/3 dans les applications de WB, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RAC1/2/3 Protéine recombinante Ag9045
• Nom complet: ras-related C3 botulinum toxin substrate 3 (rho family, small GTP binding protein Rac3)
• Masse moléculaire calculée: 192 aa, 21 kDa
• Poids moléculaire observé: 21 kDa
• Numéro d’acquisition GenBank: BC015197
• Symbole du gène: RAC3
• Identification du gène (NCBI): 5881
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Rac3 is a GTPase that belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. There are three Rac genes in mammals, which share over 90% protein identity but differ for their pattern of expression. Rac1 is ubiquitous, Rac2 is specific for hematopoietic cells, while Rac3 is prevalent in the developing nervous system. This antibody can recognize Rac1, Rac2, and Rac3.

Publications

Species	Application	Title
human	WB	Mol Cancer; F-box protein complex FBXL19 regulates TGFβ1-induced E-cadherin down-regulation by mediating Rac3 ubiquitination and degradation.; Authors - Su Dong
mouse	WB	Front Cell Neurosci; A Method to Culture GABAergic Interneurons Derived from the Medial Ganglionic Eminence.; Authors - Sira A Franchi ; KO Validated
human	WB	Mol Biol Cell; Phosphorylated cortactin recruits Vav2 guanine nucleotide exchange factor to activate Rac3 and promote invadopodial function in invasive breast cancer cells.; Authors - Brian J Rosenberg ; KD Validated
human	WB	BMC Cancer; Rac3 induces a molecular pathway triggering breast cancer cell aggressiveness: differences in MDA-MB-231 and MCF-7 breast cancer cell lines.; Authors - Gest Caroline C
mouse	WB	Glycoconj J; Gangliosides in the differentiation process of primary neurons: the specific role of GM1-oligosaccharide.; Authors - Erika Di Biase
human	WB	Asian Pac J Cancer Prev; Silencing of rac3 inhibits proliferation and induces apoptosis of human lung cancer cells.; Authors - Tie-Qin Liu ; KD Validated