Anticorps Polyclonal de lapin anti-RALB

RALB Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 12340-1-AP

Synonymes

RALB, Ras related protein Ral B

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Galerie de données de validation

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  WB analysis of Y79 using 12340-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 12340-1-AP (RALB Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 12340-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12340-1-AP (RALB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human lung using 12340-1-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 12340-1-AP (RALB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Y79 using 12340-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 12340-1-AP (RALB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 12340-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 12340-1-AP (RALB Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12340-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12340-1-AP (RALB Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary tumor using 12340-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12340-1-AP (RALB antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 12340-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12340-1-AP (RALB antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 12340-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 12340-1-AP (RALB antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon using 12340-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 12340-1-AP (RALB antibody) at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules Y79, tissu cérébral de souris, tissu cérébral humain, tissu pulmonaire de souris, tissu pulmonaire humain
• Résultats positifs en IHC: tissu de tumeur ovarienne humain, tissu de côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 2 publications below

Informations sur le produit

12340-1-AP cible RALB dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Drosophile, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RALB Protéine recombinante Ag2992
• Nom complet: v-ral simian leukemia viral oncogene homolog B (ras related; GTP binding protein)
• Masse moléculaire calculée: 206 aa, 23 kDa
• Poids moléculaire observé: 23 kDa
• Numéro d’acquisition GenBank: BC018163
• Symbole du gène: RALB
• Identification du gène (NCBI): 5899
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
Drosophila	IHC	Development; Ral GTPase promotes asymmetric Notch activation in the Drosophila eye in response to Frizzled/PCP signaling by repressing ligand-independent receptor activation.; Authors - Cho Bomsoo B
human	WB	Front Chem; Design, Synthesis, and Biological Evaluation of Pyrano[2,3-c]-pyrazole-Based RalA Inhibitors Against Hepatocellular Carcinoma.; Authors - Yuting Wang
human	WB,IHC	Arch Biochem Biophys; MicroRNA-139 inhibits pancreatic-cancer carcinogenesis by suppressing RalB via the Ral/RAC/PI3K pathway.; Authors - Yan Wang

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