Anticorps Polyclonal de lapin anti-RASAL2

• Phare
• Validé par KD/KO

RASAL2 Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(5)

Avis (1)

N° de cat : 22140-1-AP

Synonymes

nGAP, RAS protein activator like 2, RASAL2

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Galerie de données de validation

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  WB analysis of HeLa using 22140-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22140-1-AP (RASAL2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 22140-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 22140-1-AP (RASAL2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 3 publications below
• IP: See 2 publications below

Informations sur le produit

22140-1-AP cible RASAL2 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RASAL2 Protéine recombinante Ag17555
• Nom complet: RAS protein activator like 2
• Masse moléculaire calculée: 1262 aa, 142 kDa
• Poids moléculaire observé: 140-150 kDa
• Numéro d’acquisition GenBank: BC110611
• Symbole du gène: RASAL2
• Identification du gène (NCBI): 9462
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RASAL2 (Ras protein activator like 2), also known as nGAP, is a Ras GTPase-activating protein, containing a GRD domain and a coiled-coil structure at the C terminus. It is able to complement the defective RasGAP function in a yeast system. RASAL2 is highly homologous to Ras-specific GTPase activating proteins (RasGAPs). Two transcript variants exist for RASAL2 due to alternative splicing events.

Publications

Species	Application	Title
mouse	WB	Mol Metab; Rasal2 deficiency reduces adipogenesis and occurrence of obesity-related disorders.; Authors - Xiaoqiang Zhu ; KD Validated
human,mouse	WB,IHC,IF	J Clin Transl Hepatol; RASAL2 Deficiency Attenuates Hepatic Steatosis by Promoting Hepatic VLDL Secretion via the AKT/TET1/MTTP Axis; Authors - Hao Ding ; KD Validated
human	WB,IP,IHC,IF	Int J Biol Sci; RASAL2 mediated the enhancement of YAP1/TIAM1 signaling promotes malignant phenotypes of pancreatic ductal adenocarcinoma; Authors - Yangyang Yue ; KD Validated
human	WB,IP	J Transl Med; Rasal2 inhibits autophagic-exosomes secretion via regulating Rab27a in triple-negative breast cancer progression; Authors - Xuan Wang
human	IF	Transl Res; RAS protein activator-like 2 (RASAL2) initiates peritubular capillary rarefaction in hypoxic renal interstitial fibrosis; Authors - Yu Zhang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

anwesh (Verified Customer) (11-14-2019)

Clean bands observed at the right size of ~140KD in both human and mouse cell lines

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: A549, MEF's cells

RASAL2 Antibody Western Blot, validation (1:1000 dilution) in A549, MEF's cells (Cat no:22140-1-AP)

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