Anticorps Polyclonal de lapin anti-RASGRP3

• Phare
• Validé par KD/KO

RASGRP3 Polyclonal Antibody for IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, FC, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 13162-1-AP

Synonymes

GRP3, KIAA0846, Ras guanyl releasing protein 3, RAS-GRP, RASGRP3

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Galerie de données de validation

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  WB analysis using 13162-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13162-1-AP (RASGRP3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 13162-1-AP

  WB result of RASGRP3 antibody (13162-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RASGRP3 transfected PC-3 cells.

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  WB analysis of PC-3 using 13162-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 13162-1-AP (RASGRP3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of RAW 264.7 using 13162-1-AP

  IP result of anti-RASGRP3 (IP:13162-1-AP, 4ug; Detection:13162-1-AP 1:500) with RAW 264.7 cells lysate 3200 ug.

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  IHC staining of human ovary tumor using 13162-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor slide using 13162-1-AP (RASGRP3 Antibody) at dilution of 1:50.

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  IHC staining of human ovary tumor using 13162-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor slide using 13162-1-AP (RASGRP3 Antibody) at dilution of 1:50.

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Applications testées

• Résultats positifs en WB: cellules RAW 264.7, cellules PC-3, cellules Raji, cellules Ramos
• Résultats positifs en IP: cellules RAW 264.7,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 5 publications below
• FC: See 1 publications below

Informations sur le produit

13162-1-AP cible RASGRP3 dans les applications de WB, IP, IHC, FC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RASGRP3 Protéine recombinante Ag3810
• Nom complet: RAS guanyl releasing protein 3 (calcium and DAG-regulated)
• Masse moléculaire calculée: 690 aa, 78 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC027849
• Symbole du gène: RASGRP3
• Identification du gène (NCBI): 25780
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

AS guanyl releasing protein 3 (RasGRP3) is a member of the RasGRP family and is able to promote guanine nucleotide exchange of Ha-Ras, R-Ras and Rap1 (PMID: 9582122). RasGRP3 is involved in a diverse range of important biological processes. RasGRP3 has emerged as an important mediator of signaling downstream from receptor coupled phosphoinositide turnover in B and T cells. Overexpression of RasGRP3 is commonly associated with many malignancies, such as pre-Bcell leukemia, Burkitt's lymphoma and natural killer-like T-cell leukemia. Recently, it has been reported that upregulation of RASGRP3 expression in prostate cancer correlates with aggressive capabilities and predicts biochemical recurrence after radical prostatectomy (PMID: 24418912).

Publications

Species	Application	Title
human	IHC	Cancer Cell; RasGRP3 Mediates MAPK Pathway Activation in GNAQ Mutant Uveal Melanoma.; Authors - Xu Chen
human	WB,FC	BMC Med; Downregulation of AC092894.1 promotes oxaliplatin resistance in colorectal cancer via the USP3/AR/RASGRP3 axis; Authors - Zhijian Zheng ; KD Validated
human	WB,IHC	Am J Cancer Res; A somatic mutation of RasGRP3 decreases Na+/I- symporter expression in metastases of radioactive iodine-refractory thyroid cancer by stimulating the Akt signaling pathway.; Authors - Jianlu Song
human	WB,IHC	Gene; RasGRP3 controls cell proliferation and migration in papillary thyroid cancer by regulating the Akt-MDM2 pathway.; Authors - Wangwang Qiu ; KD Validated
human	WB, IHC	J Vet Sci; Production and immunogenicity of chimeric virus-like particles containing the spike glycoprotein of infectious bronchitis virus.; Authors - Lishan Lv
human	WB,IHC	Prostate Cancer Prostatic Dis; Upregulation of RASGRP3 expression in prostate cancer correlates with aggressive capabilities and predicts biochemical recurrence after radical prostatectomy.; Authors - X Zeng