Anticorps Polyclonal de lapin anti-RASSF6

• Phare
• Validé par KD/KO

RASSF6 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(14)

N° de cat : 11921-1-AP

Synonymes

Ras association domain-containing protein 6

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Galerie de données de validation

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  WB analysis of HT-29 using 11921-1-AP

  HT-29 cells were subjected to SDS PAGE followed by western blot with 11921-1-AP (RASSF6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MDA-MB-453s using 11921-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 11921-1-AP (RASSF6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 11921-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 11921-1-AP (RASSF6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 11921-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 11921-1-AP (RASSF6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-937 using 11921-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 11921-1-AP (RASSF6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 11921-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11921-1-AP (RASSF6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11921-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11921-1-AP (RASSF6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 11921-1-AP

  IP result of anti-RASSF6 (IP:11921-1-AP, 4ug; Detection:11921-1-AP 1:500) with HepG2 cells lysate 2400ug.

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  IHC staining of human ovary tumor using 11921-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11921-1-AP (RASSF6 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human prostate cancer using 11921-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11921-1-AP (RASSF6 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human prostate cancer using 11921-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11921-1-AP (RASSF6 antibody) at dilution of 1:100 (under 10x lens).

• 

  IF Staining of HeLa using 11921-1-AP

  Immunofluorescent analysis of Hela cells, using RASSF6 antibody 11921-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules HT-29, cellules COLO 320, cellules HeLa, cellules HepG2, cellules MDA-MB-453s, cellules PC-3, cellules U-937
• Résultats positifs en IP: cellules HepG2
• Résultats positifs en IHC: tissu de tumeur ovarienne humain, tissu de cancer de la prostate humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 10 publications below
• IHC: See 7 publications below
• IF: See 1 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

11921-1-AP cible RASSF6 dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RASSF6 Protéine recombinante Ag2565
• Nom complet: Ras association (RalGDS/AF-6) domain family member 6
• Masse moléculaire calculée: 369 aa, 43 kDa
• Poids moléculaire observé: 43 kDa
• Numéro d’acquisition GenBank: BC058835
• Symbole du gène: RASSF6
• Identification du gène (NCBI): 166824
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human,mouse	WB,IHC	Oncotarget; RASSF6 downregulation promotes the epithelial-mesenchymal transition and predicts poor prognosis in colorectal cancer.; Authors - Rui Zhou
human	WB	Mol Cancer; The novel RASSF6 and RASSF10 candidate tumour suppressor genes are frequently epigenetically inactivated in childhood leukaemias.; Authors - Hesson Luke B LB
		Epigenetics; RASSF6 exhibits promoter hypermethylation in metastatic melanoma and inhibits invasion in melanoma cells.; Authors - Jessica Mezzanotte
human	WB,IHC	J Genet Genomics; RASSF6-TRIM16 axis promotes cell proliferation, migration and invasion in esophageal squamous cell carcinoma.; Authors - Leilei Zheng ; KD Validated
human	WB	J Cell Physiol; MiR-496 promotes migration and epithelial-mesenchymal transition by targeting RASSF6 in colorectal cancer.; Authors - Hua Wang
human	WB,IHC	Ann Surg Oncol; Decreased expression of RASSF6 is a novel independent prognostic marker of a worse outcome in gastric cancer patients after curative surgery.; Authors - Wen Yugang Y

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