Anticorps Polyclonal de lapin anti-RB1CC1

• Phare
• Validé par KD/KO

RB1CC1 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 10069-1-AP

Synonymes

CC1, DRAGOU14, FIP200, KIAA0203, RB1 inducible coiled coil 1, RB1CC1, RBICC

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Galerie de données de validation

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  WB analysis of HEK-293 using 10069-1-AP

  WB result of RB1CC1 antibody (10069-1-AP, 1:1000) with si-Control and si-RB1CC1 transfected HEK293 cells.

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  WB analysis of MCF-7 using 10069-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10069-1-AP (RB1CC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 10069-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10069-1-AP (RB1CC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10069-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10069-1-AP (RB1CC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 10069-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10069-1-AP (RB1CC1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 10069-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10069-1-AP (RB1CC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10069-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10069-1-AP (RB1CC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10069-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10069-1-AP (RB1CC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10069-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10069-1-AP (RB1CC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10069-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10069-1-AP (RB1CC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10069-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10069-1-AP (RB1CC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10069-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10069-1-AP (RB1CC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10069-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10069-1-AP (RB1CC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10069-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10069-1-AP (RB1CC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 10069-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 10069-1-AP (RB1CC1 Antibody) at dilution of 1:50.

• 

  IHC staining of human kidney using 10069-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10069-1-AP (RB1CC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 10069-1-AP

  Immunofluorescent analysis of HeLa cells using 10069-1-AP (RB1CC1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules MCF7, cellules HEK-293, cellules HeLa, cellules NIH/3T3, tissu de muscle squelettique de souris
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

10069-1-AP cible RB1CC1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RB1CC1 Protéine recombinante Ag0003
• Nom complet: RB1-inducible coiled-coil 1
• Masse moléculaire calculée: 1594 aa, 183 kDa
• Poids moléculaire observé: 183 kDa
• Numéro d’acquisition GenBank: BC017556
• Symbole du gène: RB1CC1
• Identification du gène (NCBI): 9821
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RB1CC1, also named as RBICC, is implicated in the regulation of RB1 expression and functions as a DNA-binding transcription factor. It is a potent regulator of the RB1 pathway and a mediator that plays a crucial role in muscular differentiation. Its expression is, thus, a prerequisite for myogenic differentiation. Involved in autophagy. RB1CC1 is required for autophagosome formation. It is probably involved in the tumorigenesis of breast cancer. RB1CC1 is frequently mutated in breast cancer and shows characteristics of a classical tumor suppressor gene. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human RB1CC1.

Publications

Species	Application	Title
human	WB	Proc Natl Acad Sci U S A; 26S proteasomes become stably activated upon heat shock when ubiquitination and protein degradation increase.; Authors - Donghoon Lee
human	IF	Dev Cell; The RAB11A-Positive Compartment Is a Primary Platform for Autophagosome Assembly Mediated by WIPI2 Recognition of PI3P-RAB11A.; Authors - Claudia Puri