Anticorps Polyclonal de lapin anti-RBAP48

• Phare
• Validé par KD/KO

RBAP48 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

11

Avis (1)

N° de cat : 20364-1-AP

Synonymes

CAF 1 subunit C, CAF I 48 kDa subunit, CAF I p48, Histone binding protein RBBP4, NURF55, RBAP48, RBBP 4, RBBP4

---

Galerie de données de validation

• 

  WB analysis using 20364-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20364-1-AP (RBAP48 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 20364-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 20364-1-AP (RBAP48 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 20364-1-AP

  WB result of RBAP48 antibody (20364-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RBAP48 transfected HeLa cells.

• 

  WB analysis of HeLa using 20364-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20364-1-AP (RBAP48 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 20364-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 20364-1-AP (RBAP48 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 20364-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20364-1-AP (RBAP48 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 20364-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 20364-1-AP (RBAP48 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 20364-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 20364-1-AP (RBAP48 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 20364-1-AP

  IP Result of anti-RBAP48 (IP:20364-1-AP, 4ug; Detection:20364-1-AP 1:1000) with HeLa cells lysate 2000ug.

• 

  IHC staining of human cervical cancer using 20364-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 20364-1-AP (RBAP48 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human cervical cancer using 20364-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 20364-1-AP (RBAP48 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human cervical cancer using 20364-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 20364-1-AP (RBAP48 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 20364-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 20364-1-AP (RBAP48 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 20364-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 20364-1-AP (RBAP48 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human breast cancer using 20364-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 20364-1-AP (RBAP48 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 20364-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RBAP48 antibody (20364-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

---

Applications testées

• Résultats positifs en WB: cellules A431, cellules HEK-293, cellules HeLa, cellules HepG2, cellules Jurkat, cellules K-562, cellules L02, cellules SH-SY5Y, tissu cérébral de souris, tissu cérébral humain
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer du col de l'utérus humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 10 publications below
• IHC: See 1 publications below
• IP: See 2 publications below

Informations sur le produit

20364-1-AP cible RBAP48 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: retinoblastoma binding protein 4
• Masse moléculaire calculée: 48 kDa
• Poids moléculaire observé: 48-50 kDa
• Numéro d’acquisition GenBank: NM_005610
• Symbole du gène: RBBP4
• Identification du gène (NCBI): 5928
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RBBP4, also named as RBAP48 and CAF-I p48, belongs to the WD repeat RBAP46/RBAP48/MSI1 family. RBBP4 is a core histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. It is a component of several complexes which regulate chromatin metabolism. The antibody is specific to RBBP4.

Publications

Species	Application	Title
mouse	WB	Nat Cell Biol; LncGata6 maintains stemness of intestinal stem cells and promotes intestinal tumorigenesis.; Authors - Pingping Zhu
human	WB, IHC	J Clin Invest; ZIC2-dependent OCT4 activation drives self-renewal of human liver cancer stem cells.; Authors - Pingping Zhu
human	WB	Nat Commun; Identification of proximal SUMO-dependent interactors using SUMO-ID.; Authors - Orhi Barroso-Gomila
	WB	Carcinogenesis; circCTIC1 promotes the self-renewal of colon TICs through BPTF-dependent c-Myc expression.; Authors - Wei Zhan
human,mouse	WB	Int J Biol Sci; Quantitative proteomics of HFD-induced fatty liver uncovers novel transcription factors of lipid metabolism.; Authors - Shang Zhi ; KD Validated
human	WB	Chembiochem; Photoaffinity Labeling-Based Chemoproteomic Strategy Reveals RBBP4 as a Cellular Target of Protopanaxadiol against Colorectal Cancer Cells.; Authors - Fang-Fang Zhuo ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Katherine (Verified Customer) (09-25-2023)

Nice band

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: 293T