Anticorps Polyclonal de lapin anti-RbAp46

• Phare
• Validé par KD/KO

RbAp46 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(8)

N° de cat : 20365-1-AP

Synonymes

RBBP7, Retinoblastoma-binding protein p46, Retinoblastoma-binding protein 7, RBBP-7, Nucleosome-remodeling factor subunit RBAP46

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Galerie de données de validation

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  WB analysis using 20365-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20365-1-AP (RbAp46 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IP experiment of A431 using 20365-1-AP

  IP result of anti-RbAp46 (IP:20365-1-AP, 4ug; Detection:20365-1-AP 1:4000) with A431 cells lysate 2640 ug.

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  IHC staining of human cervical cancer using 20365-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 20365-1-AP (RbAp46 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human cervical cancer using 20365-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 20365-1-AP (RbAp46 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human breast cancer using 20365-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20365-1-AP (RbAp46 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 20365-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20365-1-AP (RbAp46 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 20365-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 20365-1-AP (RbAp46 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 20365-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 20365-1-AP (RbAp46 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of A431 using 20365-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using RbAp46 antibody (20365-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules COS-7, cellules HEK-293T, cellules HeLa
• Résultats positifs en IP: cellules A431
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer du col de l'utérus humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 7 publications below
• IHC: See 3 publications below

Informations sur le produit

20365-1-AP cible RbAp46 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: retinoblastoma binding protein 7
• Masse moléculaire calculée: 48 kDa
• Poids moléculaire observé: 52 kDa
• Numéro d’acquisition GenBank: NM_002893
• Symbole du gène: RBBP7
• Identification du gène (NCBI): 5931
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RBBP7, also named as RBAP46, belongs to the WD repeat RBAP46/RBAP48/MSI1 family. RBBP7 is a core histone-binding subunit that may target chromatin remodeling factors, histone acetyltransferases and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. It is a component of several complexes which regulate chromatin metabolism. The antibody is specific to RBBP7.

Publications

Species	Application	Title
human	WB	Cell Mol Gastroenterol Hepatol; Targeting HNRNPM Inhibits Cancer Stemness and Enhances Antitumor Immunity in Wnt-activated Hepatocellular Carcinoma.; Authors - Gui-Qi Zhu ; KD Validated
human	WB	Oncogene; HNF1B-mediated repression of SLUG is suppressed by EZH2 in aggressive prostate cancer.; Authors - Jianqing Wang
	IHC	Cancer Cell Int; Platelet-activating factor acetyl hydrolase IB2 dysregulated cell proliferation in ovarian cancer.; Authors - YingYing He
human	WB,IHC	Biochem Biophys Res Commun; PAFAH1B2 is a HIF1a target gene and promotes metastasis in pancreatic cancer.; Authors - Can Ma ; KD Validated
human	WB,IHC	Oncol Lett; RBBP7 is a prognostic biomarker in patients with esophageal squamous cell carcinoma.; Authors - Ning Yu ; KD Validated
human	WB	Oncogene; ZNF133 is a potent suppressor in breast carcinogenesis through dampening L1CAM, a driver for tumor progression; Authors - Lifang Li

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