Anticorps Polyclonal de lapin anti-p107

• Phare
• Validé par KD/KO

p107 Polyclonal Antibody for IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, ELISA

Conjugaison

Non conjugué

Publications

13

N° de cat : 13354-1-AP

Synonymes

CP107, p107, PRB1, RBL1, retinoblastoma like 1 (p107), Retinoblastoma like protein 1

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Galerie de données de validation

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  WB analysis of mouse testis using 13354-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13354-1-AP (p107 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 13354-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 13354-1-AP (p107 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 13354-1-AP

  IP Result of anti-p107 (IP:13354-1-AP, 4ug; Detection:13354-1-AP 1:500) with mouse testis tissue lysate 4000ug.

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Applications testées

• Résultats positifs en WB: tissu testiculaire de souris, cellules COLO 320
• Résultats positifs en IP: tissu testiculaire de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 13 publications below
• IP: See 1 publications below

Informations sur le produit

13354-1-AP cible p107 dans les applications de WB, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris, primate non humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: p107 Protéine recombinante Ag4146
• Nom complet: retinoblastoma-like 1 (p107)
• Masse moléculaire calculée: 1068 aa, 119 kDa
• Poids moléculaire observé: 119 kDa
• Numéro d’acquisition GenBank: BC032247
• Symbole du gène: RBL1
• Identification du gène (NCBI): 5933
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Retinoblastoma-like protein 1, also named RBL1, p107, is a member of E1A-binding proteins, which contains a A/B pocket, or pRb. Via this structure, RBL1 can bind to viral oncoproteins and other perteins containing LXCXE motif. It regulates cell proliferation via phosphorylation-sensitive interactions with E2F transcription factors and other proteins. Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression.(uniprot). It has been reported there are two transcirpts variants of RBL1, which encode a 119-kd protein and a 68-kd protein(PMID:7490090).

Publications

Species	Application	Title
mouse	WB,IP	Nat Commun; p107 mediated mitochondrial function controls muscle stem cell proliferative fates.; Authors - Debasmita Bhattacharya
human	WB	Cancers (Basel); RBL1/p107 Expression Levels Are Modulated by Multiple Signaling Pathways.; Authors - Elisa Ventura
human	WB	Front Cell Dev Biol; miR-29b-3p Increases Radiosensitivity in Stemness Cancer Cells via Modulating Oncogenes Axis; Authors - Dong Pan
human	WB	Oncotarget; Methylation of promoter of RBL1 enhances the radioresistance of three dimensional cultured carcinoma cells.; Authors - Dong Pan ; KD Validated
mouse	WB	Biochem Pharmacol; Ribociclib mitigates cisplatin-associated kidney injury through retinoblastoma-1 dependent mechanisms.; Authors - Ji Young Kim
human	WB	Mol Pharm; Synergetic and Antagonistic Molecular Effects Mediated by the Feedback Loop of p53 and JNK between Saikosaponin D and SP600125 on Lung Cancer A549 Cells.; Authors - Xiaoman Chen