Anticorps Polyclonal de lapin anti-RBL2

RBL2 Polyclonal Antibody for WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 27251-1-AP

Synonymes

P130, pRb2, Rb2, RBL2, RBR 2, retinoblastoma like 2 (p130), Retinoblastoma like protein 2

---

Galerie de données de validation

• 

  WB analysis of A549 using 27251-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 27251-1-AP (RBL2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 27251-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 27251-1-AP (RBL2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 27251-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 27251-1-AP (RBL2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

---

Applications testées

• Résultats positifs en WB: cellules A549, cellules Raji

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below

Informations sur le produit

27251-1-AP cible RBL2 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RBL2 Protéine recombinante Ag25087
• Nom complet: retinoblastoma-like 2 (p130)
• Masse moléculaire calculée: 130 kDa
• Poids moléculaire observé: 128 kDa
• Numéro d’acquisition GenBank: BC034490
• Symbole du gène: RBL2
• Identification du gène (NCBI): 5934
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RBL2, also named as Retinoblastoma-like protein 2 or p130, is a 1130 amino acid protein, which Belongs to the retinoblastoma protein (RB) family. RBL2 is a Key regulator of entry into cell division and is directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. RBL2 recruits and targets histone methyltransferases KMT5B and KMT5C, leading to epigenetic transcriptional repression and controls histone H4 'Lys-20' trimethylation. RBL2 probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. RBL2 may act as a tumor suppressor.

Publications

Species	Application	Title
mouse	WB	Biochem Pharmacol; Ribociclib mitigates cisplatin-associated kidney injury through retinoblastoma-1 dependent mechanisms.; Authors - Ji Young Kim
human	WB	Mol Pharm; Synergetic and Antagonistic Molecular Effects Mediated by the Feedback Loop of p53 and JNK between Saikosaponin D and SP600125 on Lung Cancer A549 Cells.; Authors - Xiaoman Chen
mouse	WB	Life Sci; Early-senescent bone marrow mesenchymal stem cells promote C2C12 cell myogenic differentiation by preventing the nuclear translocation of FOXO3.; Authors - Ji Che
human	WB	Nat Commun; An actin filament branching surveillance system regulates cell cycle progression, cytokinesis and primary ciliogenesis; Authors - Muqing Cao ; KD Validated