Anticorps Polyclonal de lapin anti-RBM15

• Phare
• Validé par KD/KO

RBM15 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, RIP, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

36

N° de cat : 10587-1-AP

Synonymes

One twenty two protein 1, OTT, OTT1, RBM15, RNA binding motif protein 15, SPEN

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Galerie de données de validation

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  WB analysis of HEK293 cells using 10587-1-AP

  WB result of RBM15 antibody (10587-1-AP, 1:3000) with si-control and si-RBM15 transfected HEK293 cells.

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  WB analysis of SW480 using 10587-1-AP

  SW480 cells were subjected to SDS PAGE followed by western blot with 10587-1-AP (RBM15 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of SW480 using 10587-1-AP

  SW480 cells were subjected to SDS PAGE followed by western blot with 10587-1-AP (RBM15 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10587-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10587-1-AP (RBM15 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 10587-1-AP

  IP result of anti-RBM15 (IP:10587-1-AP, 4ug; Detection:10587-1-AP 1:500) with HEK-293 cells lysate 2600 ug.

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  IHC staining of human breast cancer using 10587-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10587-1-AP (RBM15 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 10587-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10587-1-AP (RBM15 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 10587-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using RBM15 antibody (10587-1-AP) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules SW480, cellules HEK293, cellules HEK-293
• Résultats positifs en IP: cellules HEK-293,
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 9 publications below
• WB: See 28 publications below
• IHC: See 9 publications below
• IF: See 5 publications below
• IP: See 3 publications below
• CoIP: See 2 publications below
• RIP: See 1 publications below

Informations sur le produit

10587-1-AP cible RBM15 dans les applications de WB, RIP, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RBM15 Protéine recombinante Ag0938
• Nom complet: RNA binding motif protein 15
• Masse moléculaire calculée: 105 kDa
• Poids moléculaire observé: 100-107 kDa
• Numéro d’acquisition GenBank: BC006397
• Symbole du gène: RBM15
• Identification du gène (NCBI): 64783
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RNA-binding motif protein 15 (RBM15) belongs to the SPEN protein family, which have repressor function in several signaling pathway, and may bind to RNA through interaction with spliceosome components. It was first described as a 5′ translocation partner of the MAL gene in t(1,22)(p13;q13) infant acute megakaryocytic leukemia, and it functions in murine hematopoiesis through modulating Notch-induced transcriptional activation, which play crucial roles in leukemogenesis. Decreasing RBM15 levels with RNA interference could inhibit the growth and proliferation, block the cell cycle, and induce apoptosis in CML cells.

Publications

Species	Application	Title
mouse	WB	Nature; METTL3 regulates heterochromatin in mouse embryonic stem cells.; Authors - Wenqi Xu ; KO Validated
human	WB	Mol Cell; mTORC1 stimulates cell growth through SAM synthesis and m6A mRNA-dependent control of protein synthesis.; Authors - Elodie Villa
human	WB,IF,RIP,CoIP	Dev Cell; N6-methyladenosine modification of MALAT1 promotes metastasis via reshaping nuclear speckles.; Authors - Xinyu Wang ; KD Validated
human	WB	Elife; Cross-talk between PRMT1-mediated methylation and ubiquitylation on RBM15 controls RNA splicing.; Authors - Li Zhang
human	WB,IF,IP	Nucleic Acids Res; Nuclear export factor RBM15 facilitates the access of DBP5 to mRNA.; Authors - Zolotukhin Andrei S AS
mouse	IF	Cell Rep; A Pooled shRNA Screen Identifies Rbm15, Spen, and Wtap as Factors Required for Xist RNA-Mediated Silencing.; Authors - Benoit Moindrot