Anticorps Monoclonal anti-RBM15

• Phare
• Validé par KD/KO

RBM15 Monoclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain et plus (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

4

CloneNo.

4A1A4

N° de cat : 66059-1-Ig

Synonymes

One twenty two protein 1, OTT, OTT1, RBM15, RNA binding motif protein 15, SPEN

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Galerie de données de validation

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  WB analysis using 66059-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66059-1-Ig (RBM15 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

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  WB analysis of HEK293 cells using 66059-1-Ig

  WB result of RBM15 antibody (66059-1-Ig, 1:4000) with si-control and si-RBM15 transfected HEK293 cells.

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  WB analysis of HeLa using 66059-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66059-1-Ig (RBM15 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 66059-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 66059-1-Ig (RBM15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66059-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66059-1-Ig (RBM15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 66059-1-Ig

  human heart tissue were subjected to SDS PAGE followed by western blot with 66059-1-Ig (RBM15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 66059-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66059-1-Ig (RBM15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 66059-1-Ig

  IP Result of anti-RBM15 (IP:66059-1-Ig, 3ug; Detection:66059-1-Ig 1:500) with HEK-293 cells lysate 1800ug.

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  IHC staining of human breast cancer using 66059-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66059-1-Ig (RBM15 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66059-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66059-1-Ig (RBM15 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66059-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66059-1-Ig (RBM15 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 66059-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66059-1-Ig (RBM15 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 66059-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RBM15 antibody (66059-1-Ig, Clone: 4A1A4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HEK293, cellules HEK-293, cellules HeLa, cellules Jurkat, cellules K-562, cellules LNCaP, cellules MCF-7, tissu cardiaque humain, tissu cérébral humain
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 1 publications below

Informations sur le produit

66059-1-Ig cible RBM15 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: RBM15 Protéine recombinante Ag17970
• Nom complet: RNA binding motif protein 15
• Masse moléculaire calculée: 105 kDa
• Poids moléculaire observé: 107 kDa
• Numéro d’acquisition GenBank: BC006397
• Symbole du gène: RBM15
• Identification du gène (NCBI): 64783
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RNA-binding motif protein 15 (RBM15) belongs to the SPEN protein family, which have repressor function in several signaling pathway, and may bind to RNA through interaction with spliceosome components. It was first described as a 5′ translocation partner of the MAL gene in t(1,22)(p13;q13) infant acute megakaryocytic leukemia, and it functions in murine hematopoiesis through modulating Notch-induced transcriptional activation, which play crucial roles in leukemogenesis. Decreasing RBM15 levels with RNA interference could inhibit the growth and proliferation, block the cell cycle, and induce apoptosis in CML cells.

Publications

Species	Application	Title
human,mouse	WB	Nat Commun; Oxidative stress-induced FABP5 S-glutathionylation protects against acute lung injury by suppressing inflammation in macrophages.; Authors - Yuxian Guo
human	WB	Elife; Cross-talk between PRMT1-mediated methylation and ubiquitylation on RBM15 controls RNA splicing.; Authors - Li Zhang
	WB	EMBO Rep; The AS-RBM15 lncRNA enhances RBM15 protein translation during megakaryocyte differentiation.; Authors - Ngoc-Tung Tran
human	IHC	J Gene Med; The pathological tissue expression pattern and clinical significance of m6A-regulatory genes in non-small cell lung cancer.; Authors - Yujie Li