Anticorps Polyclonal de lapin anti-RBM15B

• Phare
• Validé par KD/KO

RBM15B Polyclonal Antibody for IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, RIP, IP, IHC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

10

N° de cat : 22249-1-AP

Synonymes

HUMAGCGB, HuOTT3, One twenty two protein 3, OTT3, RBM15B, RNA binding motif protein 15B

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Galerie de données de validation

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  WB analysis of HeLa using 22249-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22249-1-AP (RBM15B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 22249-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 22249-1-AP (RBM15B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 22249-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22249-1-AP (RBM15B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 22249-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22249-1-AP (RBM15B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 22249-1-AP

  IP Result of anti-RBM15B (IP:22249-1-AP, 4ug; Detection:22249-1-AP 1:500) with HeLa cells lysate 2500ug.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, tissu cérébral de souris
• Résultats positifs en IP: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 4 publications below
• CoIP: See 1 publications below
• RIP: See 2 publications below

Informations sur le produit

22249-1-AP cible RBM15B dans les applications de WB, RIP, IP, IHC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RBM15B Protéine recombinante Ag17744
• Nom complet: RNA binding motif protein 15B
• Masse moléculaire calculée: 890 aa, 97 kDa
• Poids moléculaire observé: 100-120 kDa, 70 kDa
• Numéro d’acquisition GenBank: BC001367
• Symbole du gène: RBM15B
• Identification du gène (NCBI): 29890
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RBM15B, one members of the SPEN (Split-end) family of proteins, have repressor function in several signaling pathways and may bind to RNA through interaction with spliceosome components. Present polyclonal anti-RBM15B antibody was produced by immunizing animals with C-terminus of RBM15B, which homolog with a short form of RBM15B(563aa, ~70kDa), thus this antibody can recognize full length of RBM15B(~100kDa) and splice-one(70kDa)

Publications

Species	Application	Title
mouse	WB	Nature; METTL3 regulates heterochromatin in mouse embryonic stem cells.; Authors - Wenqi Xu ; KO Validated
human	WB	Mol Cell; mTORC1 stimulates cell growth through SAM synthesis and m6A mRNA-dependent control of protein synthesis.; Authors - Elodie Villa
human	RIP	Elife; SNORD90 induces glutamatergic signaling following treatment with monoaminergic antidepressants; Authors - Rixing Lin
human	WB	eNeuro; Altered Expression of the m6A Methyltransferase METTL3 in Alzheimer's Disease.; Authors - He Huang
human	IHC	Front Genet; m6A Regulatory Gene-Mediated Methylation Modification in Glioma Survival Prediction.; Authors - Guiyun Zhang
human	IHC	Med Sci Monit; Expression and Prognostic Significance of m6A-Related Genes in Lung Adenocarcinoma.; Authors - Yingying Zhang