Anticorps Polyclonal de lapin anti-RBM24

• Phare
• Validé par KD/KO

RBM24 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, RIP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

8

N° de cat : 18178-1-AP

Synonymes

RBM24, RNA binding motif protein 24, RNA binding protein 24, RNPC6

---

Galerie de données de validation

• 

  WB analysis using 18178-1-AP

  mouse heart tissue lysates were subjected to SDS PAGE followed by western blot with 18178-1-AP (RBM24 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 18178-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18178-1-AP (RBM24 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human skeletal muscle using 18178-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 18178-1-AP (RBM24 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 18178-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 18178-1-AP (RBM24 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of mouse skeletal muscle using 18178-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using RBM24 antibody (18178-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse skeletal muscle using 18178-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using RBM24 antibody (18178-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Applications testées

• Résultats positifs en WB: tissu cardiaque de souris, cellules HeLa
• Résultats positifs en IHC: tissu de muscle squelettique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu de muscle squelettique de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 6 publications below
• RIP: See 1 publications below

Informations sur le produit

18178-1-AP cible RBM24 dans les applications de WB, RIP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RBM24 Protéine recombinante Ag12871
• Nom complet: RNA binding motif protein 24
• Masse moléculaire calculée: 191 aa, 20 kDa
• Poids moléculaire observé: 20-27 kDa
• Numéro d’acquisition GenBank: BC104808
• Symbole du gène: RBM24
• Identification du gène (NCBI): 221662
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RNA-binding proteins have a pivotal role in post-transcriptionally regulating gene expression. During early development they exhibit temporally and spatially regulated expression pattern, and affect the proteome of a single cell by controlling alternative splicing, transport, localization, and stability of their target RNAs [PMID:22510371]. Their expression are tissue-specific, and their crucial influence on the protein set-up especially in muscle cells has become recognized recently [PMID:21982546]. RBM24 is one RNA-binding protein and plays a fundamental role in regulating cardiac gene expression, sarcomeric assembly, and cardiac contractility and may thus represent a novel pathway to cardiomyopathy [PMID:20338237].

Publications

Species	Application	Title
mouse	IF	Elife; Dual expression of Atoh1 and Ikzf2 promotes transformation of adult cochlear supporting cells into outer hair cells.; Authors - Suhong Sun
mouse	IF	Development; Mosaic CRISPR-stop enables rapid phenotyping of nonsense mutations in essential genes.; Authors - Guangqin Wang
mouse	IF	Sci Rep; Rbm24 displays dynamic functions required for myogenic differentiation during muscle regeneration.; Authors - Raphaëlle Grifone ; KD Validated
mouse	IF	Dev Dyn; Expression patterns of Rbm24 in lens, nasal epithelium and inner ear during mouse embryonic development.; Authors - Raphaëlle Grifone
chicken, mouse	IF	Mech Dev; The RNA-binding protein Rbm24 is transiently expressed in myoblasts and is required for myogenic differentiation during vertebrate development.; Authors - Raphaëlle Grifone
human	IHC,RIP	J Mol Med (Berl); Mechanism of METTL14-mediated m^6A modification in non-small cell lung cancer cell resistance to cisplatin; Authors - Shulei Gong