Anticorps Polyclonal de lapin anti-RBM3

• Phare
• Validé par KD/KO

RBM3 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, RIP, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

33

N° de cat : 14363-1-AP

Synonymes

IS1 RNPL, Putative RNA binding protein 3, RBM3, RNA binding motif protein 3, RNPL

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Galerie de données de validation

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  WB analysis using 14363-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14363-1-AP (RBM3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 14363-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14363-1-AP (RBM3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 14363-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 14363-1-AP (RBM3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 14363-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 14363-1-AP (RBM3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 14363-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14363-1-AP (RBM3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MCF-7 using 14363-1-AP

  IP Result of anti-RBM3 (IP:14363-1-AP, 4ug; Detection:14363-1-AP 1:300) with MCF-7 cells lysate 3200ug.

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  IHC staining of human testis using 14363-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14363-1-AP (RBM3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 14363-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14363-1-AP (RBM3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 14363-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14363-1-AP (RBM3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 14363-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14363-1-AP (RBM3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 14363-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14363-1-AP (RBM3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 14363-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14363-1-AP (RBM3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 14363-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14363-1-AP (RBM3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung using 14363-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 14363-1-AP (RBM3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 14363-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 14363-1-AP (RBM3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 14363-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14363-1-AP (RBM3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HeLa using 14363-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 14363-1-AP (RBM3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A375, cellules HepG2, cellules Jurkat, cellules MCF-7, cellules NIH/3T3, cellules Raji
• Résultats positifs en IP: cellules MCF-7
• Résultats positifs en IHC: tissu testiculaire humain, tissu cérébral humain, tissu pulmonaire humain, tissu rénal humain, tissu splénique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 6 publications below
• WB: See 25 publications below
• IHC: See 7 publications below
• IF: See 2 publications below
• IP: See 1 publications below
• RIP: See 2 publications below

Informations sur le produit

14363-1-AP cible RBM3 dans les applications de WB, RIP, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris, squirrel
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RBM3 Protéine recombinante Ag5934
• Nom complet: RNA binding motif (RNP1, RRM) protein 3
• Masse moléculaire calculée: 17 kDa
• Poids moléculaire observé: 17-20 kDa
• Numéro d’acquisition GenBank: BC006825
• Symbole du gène: RBM3
• Identification du gène (NCBI): 5935
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RBM3, also named as RNPL, is a cold-inducible mRNA binding protein that enhances global protein synthesis at both physiological and mild hypothermic temperatures. It reduces the relative abundance of microRNAs, when overexpressed. RBM3 enhances phosphoryaltion of translation initiation factors and active polysome formation. It is up-regulated in human tumors. RBM3 is a potential proto-oncogenic proteins upon overexpression. (PMID: 19900510 ).

Publications

Species	Application	Title
mouse	WB	Nature; RBM3 mediates structural plasticity and protective effects of cooling in neurodegeneration.; Authors - Diego Peretti ; KD Validated
human,squirrel	WB	Cell; iPSCs from a Hibernator Provide a Platform for Studying Cold Adaptation and Its Potential Medical Applications.; Authors - Jingxing Ou
		EBioMedicine; The RNA-binding protein RBM3 promotes cell proliferation in hepatocellular carcinoma by regulating circular RNA SCD-circRNA 2 production.; Authors - Wei Dong
human	WB	Clin Transl Med; LncRNA BC promotes lung adenocarcinoma progression by modulating IMPAD1 alternative splicing; Authors - Qi Wen Chen
mouse	WB	J Cell Biol; Cold-induced protein RBM3 orchestrates neurogenesis via modulating Yap mRNA stability in cold stress.; Authors - Wenlong Xia ; KD Validated
mouse	WB	Front Cell Dev Biol; The RNA-Binding Protein RBM3 Promotes Neural Stem Cell (NSC) Proliferation Under Hypoxia.; Authors - Jingyi Yan

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